人工接种CMV后辣椒植株中病毒含量的时序变化及症状表型

以5份不同类型的辣(甜)椒为供试材料,人工接种CMV重花叶型株系,通过DAS-ELISA方法,分别检测接种5、10、15、20、25、30d后辣椒植株中CMV含量的时序变化,同时对供试材料进行人工接种抗性鉴定,并对2种方法得到的数据进行相关性分析。结果显示:不同类型供试材料接种5~15d后的病毒含量逐渐增加,并于15d时达到最高水平,之后略有回落;通过DAS-ELISA检测得到的植株病毒含量与人工接种抗性鉴定得到的病情指数呈正相关,说明2种鉴定方法获得的结果一致。

Temporal Change of CMV and Symptom Phenotype in Pepper after Artificial Inoculation

Taking 5 pepper of different genotypes as experimental material，after artificial inoculating heavy mosaic strain CMV，we tested the temporal change of CMV content in 5，10，15，20，25，30 days by DASELISA． At the same time，the artificial classification method was used，and we carried out analysis on the correlation of data gained through these 2 methods．The results showed that the virus content increased at 5， 10，15 days in turn，reached the highest level at 15 days．Then，the virus content reduced slightly．The virus content of the plants obtained by DAS-ELISA was positively correlated with the disease index obtained by artificial inoculation．The results obtained by the 2 methods were consistent.