多粘类芽孢杆菌L1-9菌株对番茄早疫病的抑菌防病作用

L1-9菌株是从连云港海域海泥中分离获得的1株多粘类芽孢杆菌。采用平板对峙法和打孔法测定L1-9菌株及其发酵液和无菌发酵液对番茄早疫病菌的抑制作用，结果表明：L1-9菌株和无菌发酵液对番茄早疫病菌菌丝生长有明显的抑制作用，培养4 d的抑菌带宽度分别达到17.6 mm和15.8 mm，L1-9菌株的发酵液和无菌发酵液能明显降低早疫病菌的孢子萌发率并抑制芽管的伸长，发酵液的抑制作用高于无菌发酵液。采用离体叶片法和盆栽法测定该菌株的室内防病效果，结果表明：L1-9菌株的发酵液和无菌发酵液对番茄早疫病具有较好的防治效果，发酵液的防病效果高于无菌发酵液，其离体叶片法的保护效果和治疗效果分别达到79.28 %和67.20 %。

Inhibitory Effects and Control Efficacy of Paenibacillus polymyxa Strain L1-9 on Alternaria solani of Tomato

L1-9 is a Paenibacillus polymyxa strain isolated from sediment of Lianyungang harbor. In this paper, the antimicrobial activities of L1-9 strain and its fermentations and culture filtrate against Alternaria solani of tomato（Lycopersicon esculentum Mill.）were detected through dual culture methods on PDA medium and agar-well diffusion method. The results indicated that fermentations and culture filtrate of L1-9 strain had obvious antimicrobial effects on restraining conidiophores sprouting of Alternaria solani strain. The inhibition zones got 17.6 mm and 15.8 mm, respectively, after 4 days culture. Fermentations and culture filtrate of L1-9 strain could restrain germ tube elongation of Alternaria solani strain. Moreover, fermentations had stronger antimicrobial effects than its culture filtrate. Biological control functions in laboratory were tested by 2 screening methods: detached leaf method and pot method. The results showed that fermentations of L1-9 and its culture filtrate had better control efficacies against tomato early blight. And the control efficacies of fermentations are better than culture filtrate. The protective and therapeutic effects got 79.28 % and 67.20 %, respectively.