用于黄瓜白粉病抗性鉴定的InDel标记

根据黄瓜白粉病抗性主效基因/QTL的插入/缺失突变,将其设计成共显性的In Del分子标记。通过群体连锁分析,发现In Del-MLO1标记与黄瓜白粉病抗性主效基因共分离。应用该标记对24份来自世界各地且白粉病抗性已知的黄瓜材料进行抗性验证,21份材料的抗性表型与标记的带型一致,说明在黄瓜进化和驯化过程中,造成抗病表型的该插入/缺失突变发生普遍。因此,该In Del标记适合于大多数黄瓜材料的白粉病抗性鉴定和分子标记辅助育种,从而加快黄瓜白粉病抗病育种的进程。

InDel Markers for Identification of Cucumber Powdery Mildew Resistance

The co-dominant InDel markers were successfully explored based on the insertion-deletion （InDel）mutation in the major gene/QTL conferring powdery mildew resistance in cucumber（Cucumis sativus L.）. The InDel marker InDel-MLO1 was co-segregated with the major gene for powdery mildew resistance through linkage analysis. Known of powdery mildew resistance phenotype，24 cucumber lines originated from various geographical regions of the world were tested by the InDel marker. The results showed that the genotypes of InDel-MLO1 were consistent with their resistance phenotypes in 21 cucumber lines，which indicated that this causal InDel mutation for powdery mildew resistance occurred frequently during the evolution and domestication of cucumber. Therefore，our finding confirmed that the InDel marker is very useful for identification of cucumber powdery mildew resistance and molecular marker-assisted breeding for most of the cucumber lines，and thus，the process of powdery mildew resistance breeding in cucumber will be accelerated.