辣椒茸毛性状的遗传研究和基因定位

植株表面茸毛常与抗虫、耐干旱、耐盐、抗病及防御紫外线辐射等有着直接或间接的关系,在抗性育种中有着重要的潜在利用价值。本试验以多茸毛辣椒材料1789M和无茸毛辣椒材料1788W及其F1、F2群体为试材,观察辣椒体表茸毛的形态特征及其分布特点,研究辣椒茸毛性状的遗传规律,并结合群体分离分析法(BSA)对辣椒茸毛基因进行定位。结果表明:辣椒植株茸毛是由部分表皮细胞向上突起形成,以直立不分杈的单茸毛为主,并且植株不同部位的茸毛密度不同,一般为幼茎成熟茎叶片背面叶片正面;辣椒茸毛性状由1对显性核基因控制,该基因被定位在辣椒10号染色体上,位于SSR标记ssr10-19和ssr10-21之间,且两标记与该茸毛基因的相对遗传距离分别为5.3c M和13.3c M。

Genetic Inheritance and Gene Mapping of Pepper Trichomes Traits

There is a direct or indirect relationship between plant trichomes and insect resistance，drought tolerance，salt tolerance，disease resistance and ultraviolet radiation，which has important and potential utilization values in resistance breeding．Taking high generation inbred lines of pastel‘1789M’，no pastel chili‘1788W’and their F1、F2 population as the test materials，this experiment observed the morphological characteristics，distribution characteristics of pepper surface trichomes，and studied the genetic regulation of trait and fixed the gene combining the segregation population analysis（BSA）．The results showed that the pepper trichomes are formed part of the epidermal cells jest upward，pepper is given priority to with upright no branch fuzz and density is different in different parts of the plant，generally young stem ＞ matured stem ＞ positive leaf ＞back leaf. We found a pair of dominant nuclear genes controlled pepper trichomes formation，and the pepper gene location was on chromosome 10，the SSR markers between ssr10-19 to ssr10-21．The genetic distance between these 2 markers and the trichomes gene were 5.3 cM and 13.3 cM，respectively．