| SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库 |
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| 引用本文： | 周志国,明月梅.SMART技术构建的辣椒疫霉菌与辣椒互作AD-cDNA文库[J].中国蔬菜,2015,1(11):51-54. |
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| 作者姓名： | 周志国 明月梅 |
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| 作者单位： | （廊坊师范学院生命科学学院，河北廊坊 065000） |
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| 基金项目： | 河北省科技计划项目（12226306），河北省高校食药用菌应用技术研发中心项目（YF201411） |
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| 摘 要： | 为了分离和克隆辣椒中疫霉菌诱导基因,以接种辣椒疫霉菌的叶片为材料,利用SMART技术构建辣椒疫霉菌与辣椒互作的双杂交c DNA文库。结果表明:该文库容量为3.6×106cfu,重组率88%左右,插入片段集中在300~2000bp之间,平均长度约为800bp,表明获得的文库质量较高,该文库将为分子育种提供重要的基因资源。
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| 关 键 词： | 辣椒疫霉菌 SMART 技术 双杂交cDNA 文库 |
Construction of AD-cDNA Library for Interaction of Phytophthora capsici and Pepper in SMART Technology |
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| ZHOU Zhi-guo,MING Yue-mei.Construction of AD-cDNA Library for Interaction of Phytophthora capsici and Pepper in SMART Technology[J].China Vegetables,2015,1(11):51-54. |
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| Authors: | ZHOU Zhi-guo MING Yue-mei |
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| Institution: | （Life Science Department，Langfang City Normal College，Langfang 065000，Hebei，China） |
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| Abstract: | In order to identify the Phytophthora capsici stress induced genes of pepper，a double-hybrid cDNA library of Phytophthora capsici infected pepper leaves was constructed using SMART technology. The results showed that the cDNA library contained 3.6×106 independent clones，and the recombination rate was 88%.Insert fragments ranged from 300 bp to 2 000 bp，and the average length of the library was about 800 bp. These data demonstrate that the library is qualified，and able to provide important genetic resources for molecular breeding. |
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| Keywords: | Phytophthora capsici SMART technology Double-hybrid cDNA library |
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