灰葡萄孢菌胞外大分子分泌物的植物毒性及漆酶性质鉴定

采用透析的方法提取灰葡萄孢菌分泌的大于14 kD的胞外大分子物质,将其接种到番茄和菊花叶片组织,检测灰葡萄孢菌胞外大分子分泌物的植物毒性,并以鞣酸和RB亮蓝为指示剂鉴定灰葡萄孢菌胞外大分子分泌物的漆酶性质。结果显示:灰葡萄孢菌胞外大分子分泌物可导致叶片组织坏死,具有植物毒性;接种灰葡萄孢菌后第3天,鞣酸和RB亮蓝平板出现不同程度的显色反应,第6天时平板几乎完全显色,胞外大分子物质具有漆酶性质。

Identification of Phytotoxicity and Laccase Properties of Extracellular Macromolecule Substance from Botrytis cinerea

The macromolecular substances larger than 14 kD in weight， secreted by Botryis cinerea were extracted with dialysis method. These substances were directly inoculated into the leaf blades of tomato and chrysanthemum to detect their phytotoxicity. And the laccase property of these Botryis cinerea secreta was identified using tannic acid and RB light blue as the indicator. The results showed that the extracellular macromolecule substances secreted by B. cinerea could lead to leaf tissue necrosis， suggesting that they were toxic to the plants. The results also showed that 3 days after inoculation of B. cinerea， there appeared chromogenic reaction on tannic acid and RB light blue plate. And 6 days later， the plate’s color changed almost completely，indicating that the extracellular macromolecule substance had the properties of laccase.