| 黄瓜疫霉菌生防菌的筛选及鉴定 |
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| 引用本文： | 孙莉,易图永,粟智平,王雅菲,魏润洁,董俊.黄瓜疫霉菌生防菌的筛选及鉴定[J].中国蔬菜,2015,1(6):23. |
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| 作者姓名： | 孙莉 易图永 粟智平 王雅菲 魏润洁 董俊 |
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| 作者单位： | （;1. 湖南农业大学植物保护学院，湖南长沙 410128；;2.植物病虫害生物学与防控湖南省重点实验室，湖南长沙 410128；;3.植物疾病控制与利用湖南省高校重点实验室，湖南长沙 410128；;4. 烟台出入境检验检疫局，山东烟台264000） |
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| 基金项目： | 农业部公益行业计划项目（201003004），湖南省高校创新平台开放基金项目（13K064） |
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| 摘 要： | 为了筛选对黄瓜疫霉菌有抑制作用的生防菌,采用土壤稀释法从湖南、北京等地采集的土壤样品中分离到269株放线菌和细菌,以黄瓜疫霉菌为靶标菌,通过平板对峙培养法,筛选出2株对黄瓜疫霉菌有明显拮抗作用的生防菌株。采用5倍稀释发酵液对黄瓜疫霉菌进行抑菌活性检测,放线菌X54和细菌P3的抑制率达到70%以上。通过培养形态特征观察、生理生化试验及16Sr DNA分子鉴定,放线菌X54为弗吉尼亚链霉菌(Streptomyces virginiae),细菌P3为地衣芽孢杆菌(Bacillus licheniformis)。
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| 关 键 词： | 黄瓜疫霉菌 生物防治 弗吉尼亚链霉菌 地衣芽孢杆菌 |
Screening and Identification of Bio-control Agents Against Phytophthora melonis |
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| SUN Li,YI Tu-Yong,SU Zhi-Ping,WANG Ya-Fei,WEI Run-Jie,DONG Jun.Screening and Identification of Bio-control Agents Against Phytophthora melonis[J].China Vegetables,2015,1(6):23. |
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| Authors: | SUN Li YI Tu-Yong SU Zhi-Ping WANG Ya-Fei WEI Run-Jie DONG Jun |
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| Institution: | （;1.College of Plant Protection， Hunan Agricultural University， Changsha 410128， Hunan，China； ;2.Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Plant Pests， Changsha 410128， Hunan，China； ;3.Key Laboratory of Control and Utilization of Plant Disease， College of Hunan Province， Changsha 410128， Hunan，China； ;4.Yantai Entry-exit Inspection and Quarantine Bureau，Yantai 264000，Shandong，China） |
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| Abstract: | In order to screen effective antagonistic agents to Phytophthora melonis，269 strains were isolated from collected soil in Hunan and Beijing with gradient dilution method. 2 strains showed obvious antagonistic effects to Phytophthora melonis by using agar plate dual culture，and strain X54 and strain P3 were finally screened out. The diluted 5 times filtration showed their inhibition rates were over 70%. Through observation of culture morphological characteristics，physiological and biochemical experiment，and molecular identification of 16S rDNA，we found that actinomycete X54 was Streptomyces virginiae and bacterial P3 was Bacillus licheniformis. |
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| Keywords: | Phytophthora melonis Biological control Streptomyces virginiae Bacillus licheniformis |
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