EMS 处理对大白菜种子萌发及主要生化指标的影响

为了探讨大白菜诱变育种的新方式，研究了不同浓度EMS 处理对大白菜种子萌发、幼苗生长及其主要生化指标的影响。结果表明：在0～1.0% 浓度范围内，随着EMS 浓度的增加，大白菜种子的发芽势、发芽率逐渐降低，幼苗根尖和叶缘褐化程度逐渐加剧，成活率下降，种子浸出液电导率以及种子和幼苗的MDA 含量均逐渐增加；EMS 对种子及幼苗SOD、POD 活性影响表现为低浓度促进，高浓度抑制。适宜大白菜种子诱变处理的EMS 浓度范围为0.4%～0.6%。

Effects of EMS Treatment on Germination and Biochemical Characteristics of Chinese Cabbage Seed

Taking Chinese cabbage‘Jinyu 80’and‘99-2’as experimental material，studied the effect of different EMS concentration treatments on seed germination，seedling growth，and major biochemical indexes of both seed and seedling．The results showed that within the scope of 0-1.0% concentration，the germination potential and germination rate of Chinese cabbage seeds were decreased，the seedling root tip and cotyledon edge gradually turned to brown．The seedling surviving rate was decreased gradually，and the conductivity value of seed leachate and MDA contents in seed and seedling were all increased gradually．The EMS effect on SOD and POD activities of seed and seedling were expressed as low concentration with promotion，and high concentration with inhibiton．The optimal EMS concentration for Chinese cabbage seed treatment was ranged from 0.4%-0.6%．