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| 辣椒种质的抗性基因分子标记检测 | |
| 引用本文： | 李,宁,宫丽媛,高升华,尹延旭,余楚英,王,飞,陈,财,吴,君,焦春海,姚明华.辣椒种质的抗性基因分子标记检测[J].中国蔬菜,2020,1(8):19-32. |
| 作者姓名： | 李 宁 宫丽媛 高升华 尹延旭 余楚英 王 飞 陈 财 吴 君 焦春海 姚明华 |
| 作者单位： | （;1.湖北省农业科学院经济作物研究所，蔬菜种质创新与遗传改良湖北省重点实验室，湖北武汉 430064；;2. 华中农业大学植物科学技术学院，湖北武汉 430070；;3. 华中农业大学园艺林学学院，湖北武汉 430070；;4. 湖北省农业科学院，湖北武汉 430064） |
| 基金项目： | 国家重点研发计划项目（2017YFD0101903， 2016YFE0205500）， 现代农业产业技术体系建设专项（CARS- 23-G28），湖北省自然科学基金面上项目（2019CFB577），湖北省农 业科学院人才计划项目（Q2018009，C2018003） |
| 摘 要： | 利用已发表的与辣椒抗细菌性疮痂病Bs2、Bs3基因,抗根结线虫Me1、Me3/Me4基因,抗病毒病L4、Pvr4、Tsw基因,抗白粉病PMR1基因,抗疫病Phyto5.2位点连锁的分子标记,对209份辣椒种质进行分子标记检测。结果表明,209份辣椒种质中携带Bs2基因的种质1份、携带Bs3基因的种质4份;携带Me1基因连锁标记16880-1-V2片段的纯合位点种质48份、杂合位点种质12份,携带Me3/Me4基因连锁标记片段SCAR_B94的种质1份;携带抗TMV L~4/L~4基因型的纯合抗病材料11份,L~4/L~0基因型的杂合抗病材料1份;携带抗PVY Pvr4基因连锁标记CSO片段的纯合位点种质30份,杂合位点种质8份;携带抗TSWV Tsw位点连锁标记SCAC_(568)片段的种质4份;携带PMR1基因连锁标记ZL1_1826片段的种质3份;携带Phyto5.2位点连锁标记OpD04.717片段的种质38份,初步明确了209份供试辣椒种质的抗性基因型,为辣椒抗病育种提供材料选择依据。 |
| 关 键 词： | 辣椒 抗性基因 分子标记 |
Detection of Resistance Gene of Capsicum spp. Germplasm by Molecular Markers |
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| LI Ning,GONG Li-yuan,GAO Sheng-hua,YIN Yan-xu,YU Chu-ying,WANG Fei,CHEN Cai,WU Jun,JIAO Chun-hai,YAO Ming-hua.Detection of Resistance Gene of Capsicum spp. Germplasm by Molecular Markers[J].China Vegetables,2020,1(8):19-32. | |
| Authors: | LI Ning GONG Li-yuan GAO Sheng-hua YIN Yan-xu YU Chu-ying WANG Fei CHEN Cai WU Jun JIAO Chun-hai YAO Ming-hua |
| Institution: | （;1.Hubei Key Laboratory of Vegetable Germplasm Enhancement and Genetic Improvement，Cash Crops Research Institute，; Hubei Academy of Agricultural Sciences，Wuhan 430064，Hubei，China；;2.College of Plant Science and Technology，; Huazhong Agricultural University，Wuhan 430070，Hubei，China；;3.College of Horticulture and Forestry Sciences，Huazhong; Agricultural University，Wuhan 430070，Hubei，China；;4.Hubei Academy of Agricultural Sciences，Wuhan 430064，Hubei，; China） |
| Abstract: | Totally 209 Capsicum germplasm were identified by molecular markers，which linked to pepper bacterial resistant scab Bs2，Bs3 gene，root-knot nematode resistant Me1，Me3/Me4 gene，virus disease resistant L4，Pvr4，Tsw gene，powdery mildew resistant PMR1 gene and epidemic disease resistant Phyto5.2 loci．The results showed that there were only one pepper germplasm carrying Bs2 gene；4 carrying Bs3 gene； 48 carrying Me1 gene with linked marker 16880-1-V2 fragment isozygoty loci and 12 with heterozygous loci； and one carrying Me3/Me4 gene with linked marker fragment SCAR_B94；11 carrying homozygous disease resistant material TMV L4/L4 genotype；1 carrying heterozygous disease resistant material L4/L0 genotype；30 carrying PVY Pvr4 resistant gene with linked marker CSO fragment isozygoty loci and 8 heterozygous loci；4 carrying Tsw resistance locus with linked marker SCAC568 fragment；3 carrying PMR1 gene with linked marker ZL1_1826 fragment；and 38 carrying Phyto5.2 locus with linked marker OpD04.717 fragment．This study has identified preliminarily the genotypes of 209 pepper germplasm resources and provided basis for selecting disease resistant pepper materials． |
| Keywords: | Pepper Resistance gene Molecular marker |
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