番茄斑萎病毒病抗性种质筛选及人工接种鉴定方法优化

对154份番茄材料(包括普通番茄76份,樱桃番茄78份)进行两年两次田间番茄斑萎病毒病的病情指数调查,筛选出14份对番茄斑萎病毒病具有稳定抗性的番茄材料,利用sw-5-2共显性SCAR标记对田间表现抗病的材料进行分子标记鉴定,发现3份抗病材料携带抗番茄斑萎病毒病的Sw-5基因。为缩短番茄斑萎病毒病人工接种鉴定周期,以含有sw-5的抗病材料H8和感病材料M82为研究对象,设置4、6、8、10片真叶4个接种时期,分别在接种后14、21、28 d进行病情指数调查和抗性分级,结果表明,6片真叶期接种,接种后28 d进行病情调查即可有效鉴别植株番茄斑萎病毒病抗性,与8、10片真叶期接种效果相同,人工接种抗病性鉴定效率显著提升。

Screening of Resistant Germplasm of Tomato spotted wilt virus and Optimization of Artificial Identification Method

In this study，154 tomato materials，including 76 common tomatoes and 78 cherry tomatoes were investigated twice for the disease indexes of Tomato spotted wilt virus（TSWV）in fields for 2 years， and 14 of them were screened with stable resistance to TSWV．Molecular marker identification was carried out by Sw-5 co-dominant SCAR marker on materials with disease resistance in fields．Three materials carrying Sw-5 gene against TSWV were discovered．In order to shorten the artificial inoculation identification period for TSWV，this study took disease resistant material H8 containing Sw-5 and susceptible material M82 as study object；set up 4 inoculation periods for 4，6，8 and 10 true leaf blades；and conducted disease index investigation and resistance grading 14，21，28 days after inoculation．The results showed that inoculation during 6 true leaf stage，and conducting disease investigation 28 days after inoculation could effectively identify plant resistance for TSWV．Matching with the effect of inoculation in 8，10 true leaf blades stage，the efficiency of artificial inoculation for disease resistance identification was significantly promoted．