| 草莓果胶裂解酶RNA干扰表达载体构建 |
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| 引用本文： | 钱春,何桥,翟军鹏,宋波,夏蓓蓓,张兴国,梁国鲁.草莓果胶裂解酶RNA干扰表达载体构建[J].中国蔬菜,2010,1(12):26-30. |
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| 作者姓名： | 钱春 何桥 翟军鹏 宋波 夏蓓蓓 张兴国 梁国鲁 |
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| 作者单位： | 西南大学园艺园林学院，重庆400715 |
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| 基金项目： | 重庆市科委自然科学基金，中央高校基本科研业务费专项 |
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| 摘 要： | 为提高草莓果实硬度,延长货架期,利用草莓果胶裂解酶(PL)382bp的一段保守序列构建了RNA干扰植物表达载体;利用农杆菌介导将其成功转入到草莓中,通过PCR检测出2株阳性转化植株。
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| 关 键 词： | 草莓 果胶裂解酶 RNA干扰 遗传转化 |
| 收稿时间： | 2010-02-11; |
Construction of RNAi Expression Vector of Pectatelyase from Strawberry |
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| QIAN Chun,HE Qiao,ZHAI Jun-peng,SONG Bo,XIA Bei-bei,ZHANG Xing-guo,LIANG Guo-lu.Construction of RNAi Expression Vector of Pectatelyase from Strawberry[J].China Vegetables,2010,1(12):26-30. |
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| Authors: | QIAN Chun HE Qiao ZHAI Jun-peng SONG Bo XIA Bei-bei ZHANG Xing-guo LIANG Guo-lu |
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| Institution: | College of Horticulture and Landscapal, Southwest University, Chongqing 400715, China |
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| Abstract: | In order to enhance the firmness of strawberry（Fragaria ananassa Duch.）and extend the shelf life, this experiment constructed RNA interference（RNAi）expression vector using a 382 bp conservative fragment of pectate lyase. The target gene was successfully transferred into strawberry via Agrobacterium. Two postitive transformants were detected through PCR analysis. |
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| Keywords: | Pectate lyase RNAi Genetic transformation |
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