北京地区南瓜病毒病病原种类鉴定

为明确北京地区南瓜病毒病种类及其主要侵染病原,2016~2017年在北京周边采集疑似感染病毒的南瓜病样84份,并根据南瓜上的6种病毒特异性引物对其进行反转录PCR(RT-PCR)检测。结果表明:共有79份南瓜病样检测显示阳性,其中黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为52.38%;其次是小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)和西瓜花叶病毒(Watermelon mosaic virus,WMV),检出率分别为44.01%、14.29%,其他病毒暂未检出。此外,16.67%的样品受2种病毒复合侵染,CMV和ZYMV复合侵染占7.14%,ZYMV和WMV复合侵染占9.52%。北京地区南瓜上优势病毒种类为CMV,且存在病毒复合侵染现象。

Identification of Pathogen Species of Virus Diseases Infecting Squash in Beijing Area

In order to clear and definite species of squash virus diseases in Beijing area and their main infection pathogens，this paper collected 84 squash samples with suspected virus-like symptoms during 2016-2017 from Beijing area，and carried out detection by RT-PCR used 6 pairs of specific virus primers．The results showed that 79 samples were positive，among which Cucumber mosaic virus （CMV） had the highest detection rate -52.38%，followed by Zucchini yellow mosaic virus（ ZYMV） and Watermelon mosaic virus（ WMV）．Their detection rate were 44.01% and 14.29%，respectively．Other viruses were not yet detected．In addition，16.67% samples were mix-infected by 2 kinds of viruses．Mix infection of CMV and ZYMV took 7.14%．Mix infection of ZYMV and WMV took 9.52%．CMV was the prevalent pathogenic virus in Beijing area，where the mix infection of viruses on squash was existed．