大白菜BrAOP2 基因的遗传多样性及其与硫甙含量的相关性分析

以大白菜品种Chiifu 为试验材料， 采用RT-PCR 技术， 克隆了3 个硫甙合成关键基因
BrAOP2 基因的cDNA 序列（BrAOP2.1、BrAOP2.2、BrAOP2.3）。通过氨基酸同源性比对分析，BrAOP2.1
与BrAOP2.2 的同源性为78%，BrAOP2.1 与BrAOP2.3 的同源性为74%，BrAOP2.2 与BrAOP2.3 的同源
性为81%。通过32 份大白菜重测序数据分析了3 个BrAOP2 基因CDS 序列的遗传多样性，并对其与硫甙
含量的相关性进行了分析。结果表明：在32 份大白菜中共检测到7 种硫甙，其中4-戊烯基硫甙（GBN）
含量和2-羟基-3-丁烯基硫甙（PRO）含量较高，分别占总硫甙含量的23.99% 和23.16%。在BrAOP2.1
基因编码区检测到5 个变异位点，在BrAOP2.2 基因编码区检测到7 个变异位点，在BrAOP2.3 基因编码
区仅检测到1 个变异位点。其中BrAOP2.1 基因的A1051G 变异位点与PRO 含量极显著相关，BrAOP2.2
基因的A560G、C753T、A790G 和T927C 变异位点与PRO 含量显著相关，BrAOP2.2 基因的G825A 位点
与4-羟基-3-吲哚基甲基硫甙（4OH）含量显著相关。

Genetic Diversity of BrAOP2 and Analysis of Its Correlation with Glucosinolates Content in Chinese Cabbage

In this study，full length cDNA sequence of 3 BrAOP2 genes（BrAOP2.1，BrAOP2.2，
BrAOP2.3）was cloned from Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour） Olsson〕
‘Chiifu’．Sequence alignment showed that BrAOP2.1 shares 78% identity with BrAOP2.2 and 74% with
BrAOP2.3，while BrAOP2.2 and BrAOP2.3 share 81% identity．Glucosinolates profile and contents
were evaluated for 32 Chinese cabbage accessions． 7 glucosinolates were detected by HPLC. Glucobrassicanapin（
GBN） and Progoitrin（ PRO） were the most dominant glucosinolates with the highest ratio
around 23.99% and 23.16% of the total glucosinolates，respectively．Among the 32 accessions，13
SNPs were identified for BrAOP2．The correlation analysis between identified BrAOP2 SNPs and glucosinolates
content revealed that A1051G site of BrAOP2.1 and A560G，C753T，A790G and T927C
of BrAOP2.2 had significant correlation with the PRO content，while the G825A site of BrAOP2.2 had significant correlation with 4-hydroxyglucobrassicin（4OH）conten t.