| 野生番茄LA2157中热稳定抗根结线虫基因Mi-9候选基因的分离与过表达载体构建 |
| |
| 引用本文： | 王银磊,陈伟男,赵丽萍,周蓉,宋刘霞,余文贵,赵统敏.野生番茄LA2157中热稳定抗根结线虫基因Mi-9候选基因的分离与过表达载体构建[J].中国蔬菜,2017,1(12):30-34. |
| |
| 作者姓名： | 王银磊 陈伟男 赵丽萍 周蓉 宋刘霞 余文贵 赵统敏 |
| |
| 作者单位： | 1. 江苏省农业科学院蔬菜研究所，江苏南京 210014；;2. 江苏省高效园艺作物遗传改良重点实验室，江苏南京 210014；;3. 扬州大学园艺与植物保护学院，江苏扬州 225009 |
| |
| 基金项目： | 国家自然科学青年基金项目（31401884），江苏省自然科学青年基金项目（BK20140739） |
| |
| 摘 要： | 根结线虫是番茄上的重要土传病害,选育抗根结线虫品种是最有效的防治方法,但是目前转育到栽培番茄中的抗根结线虫基因Mi-1在土温高于28℃时就丧失了抗性。本试验利用热稳定抗根结线虫野生番茄材料LA2157,根据Mi-1基因的序列信息,对其中热稳定抗根结线虫Mi-9基因进行同源克隆,在LA2157中共获得2个候选基因片段;通过In-Fusion克隆技术,将候选基因与过表达载体p BI121进行连接,经电泳检测和测序分析,最终构建Mi-9候选基因的过表达重组载体。
|
| 关 键 词： | 番茄 根结线虫 Mi-9 基因 同源克隆 过表达载体构建 |
Isolation of Heat-stable Candidate Gene Mi-9 for Resistance to Root-knot Nematode in Wild Tomato LA2157 and Construction of Over-expressive Vector |
| |
| WANG Yin-lei,CHEN Wei-nan,ZHAO Li-ping,ZHOU Rong,SONG Liu-xia,YU Wen-gui,ZHAO Tong-min.Isolation of Heat-stable Candidate Gene Mi-9 for Resistance to Root-knot Nematode in Wild Tomato LA2157 and Construction of Over-expressive Vector[J].China Vegetables,2017,1(12):30-34. |
| |
| Authors: | WANG Yin-lei CHEN Wei-nan ZHAO Li-ping ZHOU Rong SONG Liu-xia YU Wen-gui ZHAO Tong-min |
| |
| Institution: | 1. Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，Jiangsu，China；;2. Key;
Laboratory for High Efficient Horticulture Crops Genetic Improvement of Jiangsu Province，Nanjing 210014，Jiangsu，;
China；;3. School of Horticulture and Plant Protection，Yangzhou University，Yangzhou 225009，Jiangsu，China |
| |
| Abstract: | Root-knot nematode disease is one of the important tomato soil-borne diseases．Breeding varieties
with root-knot nematode resistance is an effective control method to deal with this disease．But at present，Mi-1
the only gene transferred to tomato culture with resistance to root-knot nematode loses its resistance when the soil
temperature is over 28 ℃．In this study，wild tomato accession LA2157 with heat-stable resistance to root-knot
nematode was used．According to the sequence information of Mi-1，we carried out homologous cloning on Mi-9
gene with heat-stable resistance to root-knot nematode and gained 2 candidate gene segments from LA2157．The
candidate gene was ligated with the overexpression vector pBI121 by In-Fusion cloning technique．Electrophoresis
and sequencing analysis proved that the recombinant vectors of the candidate gene were constructed． |
| |
| Keywords: | Tomato Root-knot nematode Mi-9 gene Homologous cloning Over-experessive vector construction |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 |
