基于SCoT 标记的芥菜种质遗传多样性与指纹图谱

利用SCoT 标记对46 份芥菜种质资源进行遗传多样性分析。从70 条引物中筛选出21 条用于PCR 扩增，共扩增出200 条条带，其中多态性条带140 条，多态性比率为70%。供试材料相似系数介于0.77～0.95 之间，平均Nei’s 基因多样性为0.198，平均Shannon 信息指数为0.301，平均多态信息含量为0.822 7。利用UPGMA 构建46 份芥菜种质资源的聚类树状图，在相似系数D1=0.780 处，可以将46 份芥菜种质分为两大类；而在D2=0.807 处，第Ⅱ类可进一步细分为5 个亚类。选出清晰的16 个多态性位点，构建46 份芥菜种质的SCoT 指纹图谱，结果由引物SP4、SP19、SP20、SP23、SP30 所构建的DNA 指纹图谱可将供试材料完全区分开。

Genetic Diversity and Fingerprint Based on SCoT Markers in Brassica juncea （L.） Czern. et Coss.

SCoT markers were used to analyze genetic diversity of 46 mustard accessions．21 primers were screened from 70 SCoT primers and 200 SCoT bands were obtained，including 140 polymorphic bands，with a polymorphism rate of 70%．The genetic similarity coefficient values among these accessions were ranged between 0.77 to 0.95，and the average Nei’s s gene diversity（ H），Shannon’s information index（ I） and Polymorphism information content（ PIC） were 0.198，0.301 and 0.822 7，respectively.The cluster analysis conducted with UPGMA showed that 46 mustard accessions were divided into 2 groups in D1=0.780，and the group Ⅱ were divided into 5 subgroups in D2=0.807．Besides，a SCoT fingerprint of 46 mustard accessions was established with 16 sites of the 140 amplified polymorphic bands．The results showed that 46 mustard accessions can be identified by SCoT fingerprint performed with primers SP4，SP19，SP20，SP23 and SP30.