贵州野生枇杷资源遗传多样性的ISSR分析

采用ISSR标记,构建了贵州39份野生枇杷种质的DNA指纹图谱,并对其遗传多样性进行评价。结果表明,以筛选出的15条引物进行PCR扩增,获得清晰、重复性好的谱带125条,其中多态性谱带108条,多态性比例为86.4%;应用引物815、825和841的组合,构建了39份枇杷种质的ISSR指纹图谱,可以有效区分所有供试材料;15条引物的谱带数据聚类分析表明,供试种质的遗传相似性系数为0.40～0.98,在相似性系数为0.70时,可将供试种质分成五大类;聚类结果与种质表型相关,与地理分布的相关性不明显。

Genetic Diversity Analysis of Wild Loquat (Eriobotrya japonica) Germplasms from Guizhou Province Using ISSR Markers

ISSR markers were used to study the fingerprinting patterns and the genetic diversity of 39 accessions of wild loquat germplasms of Guizhou province.By using fifteen selected primers,a total of 125 clear and reproducible bands were amplified,among which 108 bands were polymorphic,accounting for 86.4% of the total.The ISSR fingerprinting patterns of 39 loquat accessions were established by using primer combinations of 815,825 and 841 that were capable of distinguishing all the accessions.The genetic diversities of the studied genotypes were revealed by UPGMA clustering of the scores of ISSR bands.Five groups were obtained from the dendrogram taking 0.70 as a threshold.The clustering result was related to germplasm phenoptypes,but not obviously to geographic distribution.