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小兴安岭红松原始林的遗传多样性研究
引用本文:郭兴,张巍,任广明.小兴安岭红松原始林的遗传多样性研究[J].森林工程,2012,28(1):14-16.
作者姓名:郭兴  张巍  任广明
作者单位:黑龙江省伊春林业科学院,黑龙江伊春,153000
基金项目:黑龙江省森林工业总局应用研究项目
摘    要:通过39个红松植株的DNA进行10个引物的ISSR实验,得出71个条带,其中特异性条带为55个,特异性位点百分率77.46%,平均每个引物产生5.5个特异性条带。可见,利用ISSR分子标记鉴别红松DNA多态性,多态性高,扩增的片段多,可用于红松种群间的亲缘关系分析研究。

关 键 词:ISSR分子标记  红松  遗传多样性

Research on Genetic Diversity of Original Pinus koraiensis in Xiaoxinganling Mountain
Guo Xing,Zhang Wei,Ren Guangming.Research on Genetic Diversity of Original Pinus koraiensis in Xiaoxinganling Mountain[J].Forest Engineering,2012,28(1):14-16.
Authors:Guo Xing  Zhang Wei  Ren Guangming
Institution:Yichun Academy of Forestry of Heilongjiagn Province, Yichun, Heilongjiang Province 153000
Abstract:ISSR test of 10 experimental primers was conducted to the DNA of 39 pine plants in this study. Results show that 71 bands are obtained, including 55 specific bands, with 77.46% specific site, meaning that the average production of each primer is 5.5 specific bands. Therefore, it can be seen that ISSR marker can be used to identify DNA polymorphism, with high polymorphism aind more amplified fragment, it can analyze the genetic relationship among populations.
Keywords:ISSR marker  Pinus koraiensis  genetic diversity
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