Karyotype analysis of interspecific fusants of basidiomycetes by pulsed-field gel electrophoresis |
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| Authors: | Takeshi Yokono Yutaka Tamai Tomonori Azuma Yoh Sakuma Kiyoshi Miura Yasuo Kojima Masahide Sunagawa Masatake Ohmasa |
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| Institution: | (1) M&S Instruments Trading Inc., 113 Yarai-cho, Shinjuku-ku, 162-0805 Tokyo, Japan;(2) Division of Environment and Resource, Graduate School of Agriculture, Hokkaido University, 060-0809 Sapporo, Japan;(3) Forest Products Research Institute, 071-0181 Asahikawa, Japan;(4) Biological Resources Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 305-8686 Ibaraki, Japan;(5) Forestry and Forest Products Research Institute, 305-8687 Ibaraki, Japan;(6) Department of Biological Resource, Faculty of Agriculture, 399-4598 Nagano, Japan |
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| Abstract: | The purpose of this research was to analyze the karyotype of the interspecific fusants of twoPleurotus species. Auxotrophic mutants derived from the cultivated strain ofP. ostreatus andP. cornucopiae were used. Protoplasts were fused electrically, and the fusants were selected under auxotrophic complementation. Esterase isozyme analysis showed that several fusants had isozyme bands originating from both parental strains, and others had unilateral isozyme bands. The fusant that had expressed isozyme bands of both parental strains showed chromosomal DNA bands of both of the parental strains in pulsedfield gel electrophoresis analysis. Despite the above results, the chromosomal composition of the fusants obtained by the pulsed-field gel electrophoresis did not exhibit all of the bands of both fusion parents. |
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| Keywords: | Pleurotus species Protoplast fusion Electrophoretic karyotype Chromosome Mushroom |
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