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| 吉林省半干旱区小干松引种栽培试验及抗旱种源的ISSR分析 | |
| 引用本文： | 周淑梅,陈建军,矫式奎,刘永富,张金福,于安,张东.吉林省半干旱区小干松引种栽培试验及抗旱种源的ISSR分析[J].吉林林业科技,2007,36(3):1-7,27. |
| 作者姓名： | 周淑梅 陈建军 矫式奎 刘永富 张金福 于安 张东 |
| 作者单位： | 1. 双辽市林业局,吉林,四平,136400 2. 吉林省林业科学研究院,吉林,长春,130033 3. 抚松县林业局,吉林,白山,134500 |
| 摘 要： | 在小干松抗旱地理种源选择的基础上,采用抗旱种源在吉林省半干旱区的多个典型立地进行了比较栽培试验研究。结果表明,小干松更适宜于在沙荒地上营造。通过多种造林方法比较,总结出小干松沙地造林实用技术。利用ISSR-PCR方法对抗旱种源进行了基因多态分析,依据扩增结果进行遗传距离分析,构建分子树状图,探讨了小干松抗旱种源间的遗传关系和利用ISSR分析进行种源鉴定的可行性。 |
| 关 键 词： | 半干旱区 小干松 引种栽培 ISSR分析 |
| 文章编号： | 1005-7129（2007）03-0001-07 |
| 收稿时间： | 2006-11-27 |
| 修稿时间： | 2006-11-27 |
Introduction test and ISSR analysis of Pinus contorta in semi-dry region of Jilin Province, China |
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| ZHOU Shu-mei,CHEN Jian-jun,JIAO Shi-kui,LIU Yong-fu,ZHANG Jin-fu,YU An,ZHANG Dong.Introduction test and ISSR analysis of Pinus contorta in semi-dry region of Jilin Province, China[J].Jilin Forestry Scicnce and Technology,2007,36(3):1-7,27. | |
| Authors: | ZHOU Shu-mei CHEN Jian-jun JIAO Shi-kui LIU Yong-fu ZHANG Jin-fu YU An ZHANG Dong |
| Abstract: | Seven drought-resistance provenances were cultivated in 3 typical locality of middle-western and semi-dry region of Jilin province China,based on provenance selection of lodgepole pine(Pinus contorta),Its gene polymorphism were determined by using ISSR marker assays,It was indicated that lodgepole pine more appropriate grows in sand,planting technique of lodgepole pine was affirmed by compared with 4 planting methods.The gene polymorphism analysis of drought-resistance provenance was conducted by using ISSR-PCR method.According to DNA amplification,the genetic distances was determined and analyzed,the molecular dendrogram was constructed.The heredity relations among drought-resistance provenances and the feasibility of provenance identification by ISSR analysis were discussed in this paper. |
| Keywords: | semi-dry region lodgepole pine introduction ISSR analysis |
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