| 用随机扩增多态DNA (RAPD)技术分析野生与笼养绿孔雀种群的遗传多样性 |
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| 引用本文： | 柯亚永,常弘,张国萍.用随机扩增多态DNA (RAPD)技术分析野生与笼养绿孔雀种群的遗传多样性[J].林业研究,2004,15(3). |
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| 作者姓名： | 柯亚永 常弘 张国萍 |
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| 作者单位： | 广东省野生动植物保护管理办公室，中山大学生命科学学院，中山大学生命科学学院 广州510173，中国，广州510275，中国，广州510275，中国 |
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| 摘 要： | 利用随机扩增多态DNA(RAPD)技术对野生14个和笼养18个绿孔雀(Pavomuticus)个体进行了种群遗传多样性分析。用23个随机引物对野生与笼养绿孔雀基因组DNA扩增,分别获得161和166个扩增片段。计算发现野生与笼养绿孔雀的种群内平均相对遗传距离分别是0.0555和0.1355,两种群间的平均相对遗传距离为0.1635;野生绿孔雀和笼养绿孔雀种群的Shannon多样性指数平均分别是0.4348和1.0163,表明有显著性差异。以上分析都显示野生绿孔雀的遗传多样性很低。利用UPGMA法聚类显示两个种群都是分别来源于两个家系,可据此进行绿孔雀的繁育管理。图1表3参5。
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| 关 键 词： | 绿孔雀 基因组DNA 随机扩增多态DNA(RAPD) |
Analysis of genetic diversity for wild and captive green peafowl populations by random amplified polymorphic DNA technique |
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| KE Ya-yong,CHANG Hong,ZHANG Guo-ping Wild Fauna and Flora Protection and Management Office,Guangdong Forestry Bureau,Guangzhou ,P. R. China School of Life Science,Zhongshan University,Guangzhou ,P. R. China.Analysis of genetic diversity for wild and captive green peafowl populations by random amplified polymorphic DNA technique[J].Journal of Forestry Research,2004,15(3). |
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| Authors: | KE Ya-yong CHANG Hong ZHANG Guo-ping Wild Fauna and Flora Protection and Management Office Guangdong Forestry Bureau Guangzhou P R China School of Life Science Zhongshan University Guangzhou P R China |
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| Institution: | KE Ya-yong1,CHANG Hong2,ZHANG Guo-ping2 1 Wild Fauna and Flora Protection and Management Office,Guangdong Forestry Bureau,Guangzhou 510173,P. R. China 2 School of Life Science,Zhongshan University,Guangzhou 510275,P. R. China |
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| Abstract: | The genetic diversity of the populations for 14 wild green peafowls (Pavo muticus) and 18 captive green pea-fowls was investigated by using the technology of random amplified polymorphic DNA (RAPD). Totally 161 and 166 ampli-fied bands were obtained by using 23 arbitrary primers to amplify the genomic DNA of wild and captive green peafowls re-spectively. The results showed that the average relative genetic distance of the wild and captive green peafowls popula-tions was 0.0555 and 0.1355, respectively, and difference of the average relative genetic distances between the two popu-lations was 0.1635. The Shannon diversity index for the wild and captive green peafowl populations was 0.4348 and 1.0163, respectively, which means that there exists significant difference in genetic diversity between the two populations, and the genetic diversity of wild green peafowl was low. The two populations originated from two different families according to analysis by the UPGMA method. This research can provide the theoretical basis for supervising genealogies management of peafowl populations. |
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| Keywords: | Green peafowl Pavo muticus Genomic DNA Random amplified polymorphic DNA (RAPD) |
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