秋茄内生真菌新纪录种(英文)

红树林内生真菌1893是从中国南海秋茄(Kandelia candel)上分离的一株内生真菌,在液体发酵条件下能够产生包括1893A和1893B在内的多种新物质。为了正确的鉴定该菌株并为进一步的工业应用做准备,本文结合形态学和分子学两方面对它进行了鉴定,首先,在纯培养条件下,将它和具有以下形态特征的相似菌株进行比较:产生受精丝或像受精丝一样的菌丝,这些菌丝又和像雄器一样的菌丝发生明显的融合现象;环状产囊体与大量菌丝缠绕形成菌核前体。进一步通过该菌株在寄主秋茄和非寄主芒果叶子上的培养性状与其它相似真菌比较,结果将该菌株归于拟茎点霉属(Phomopsis)。其次,在ITS序列水平上,将它和具有形态学或生态学相似性的拟茎点霉进行系统发育分析。最后,综合形态学和分子学,红树林内生真菌1893被定为Diaporthe phaseolorum var.sojae,本文是首次报道Diaporthe phaseolorum var.sojae做为内生真菌寄生于秋茄。图5参43。

First report of an endophyte (Diaporthe phaseolorum var. sojae) from Kandelia candel

Mangrove endophytic fungus 1893 was isolated from Kandelia candel from an estuarine mangrove on the South China Sea Coast. Two new lactones 1893A and 1893B, together with other known compounds, have been isolated from its fermentation broth. To classify the endophyte correctly for further industrial application, a combination of morphological and molecular techniques was used to approach its identity. The endophyte was compared with similar species having trichogynes or trichogyne-like hyphae which apparently fused with an-theridium-like hyphae, and perithecia initials developing from an ascogonial coil surrounded by enveloping hyphae in early developmental stages on pure culture. Further morphological characteristics on host and non-host were used for comparison with similar species when the endophyte was cultivated on leaves of Kandelia candel and Mangifera indica, respectively, which resulted in classifying the endophyte as a Phomopsis species. The ITS sequence of rDNA was used to infer its phylogenetic relationships with Phomopsis species that resembled the strain in morphology or ecology. Finally, the endophyte was identified as Diaporthe phaseolorum var. sojae based on morphological and molecular evidence. Our study is a first report of Diaporthe phaseolorum var. sojae isolated from mangrove Kandelia candel.