| 朝仓花椒组织培养快繁试验 |
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| 引用本文： | 郭伟珍,林艳,毕君,赵京献,徐振华,王春荣,王超.朝仓花椒组织培养快繁试验[J].林业科技开发,2005,19(5):19-21. |
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| 作者姓名： | 郭伟珍 林艳 毕君 赵京献 徐振华 王春荣 王超 |
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| 作者单位： | 河北省林业科学研究院 |
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| 基金项目： | 国家“948”项目“日本花椒优良品种及丰产无公害栽培技术引进”. |
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| 摘 要： | 以朝仓花椒为试材，研究了其组培快繁技术。试验表明，4～5月份接种外植体易获得无菌苗；初期适宜的增殖培养基为MS＋6-BA1．0mg／L（单位下同）＋IBA0．2＋3．0％蔗糖，继代增殖培养基为MS＋6-BA0．5＋IBA0．1＋3．0％蔗糖，适宜的生根培养基为1／4MS＋NAA0．3＋2．5％蔗糖，移栽最佳基质为蛭石。
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| 关 键 词： | 朝仓花椒 组织培养 快速繁殖 MS培养基 移栽 快繁试验 花椒 增殖培养基 组培快繁技术 生根培养基 |
| 收稿时间： | 2005-05-31 |
| 修稿时间： | 2005-06-25 |
Study on Tissue Culture of Zanthoxylum piperitum DC var. inerme Makino |
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| Guo wei-zhen,LIN Yan,BI Jun,Zhao jing-xian,XU Zhen-hua,WANG Chun-rong,WANG Chao.Study on Tissue Culture of Zanthoxylum piperitum DC var. inerme Makino[J].China Forestry Science and Technology,2005,19(5):19-21. |
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| Authors: | Guo wei-zhen LIN Yan BI Jun Zhao jing-xian XU Zhen-hua WANG Chun-rong WANG Chao |
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| Abstract: | The tissue culture and rapid propagation of Zanthoxylum piperitum DC var.inerme Makino were studied in this paper.The results indicated that sterile regenerated plants can be easy cultivated from explants during April and May.The optimum proliferation medium was MS+1.0 mg/L 6-BA+0.2 mg/L IBA+3%Sugar.The best subculture and rooting media were MS+0.5 mg/L 6-BA+0.1 mg/L IBA+3.0%Sugar and 1/4 MS+0.3 mg/L NAA+2.5%Sugar,respectively.The result also showed that vermiculite was the best growing medium for plantlets from tissue culture among all the designed medium. |
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| Keywords: | Zanthoxylurn piperiturn DC var inerme Makino Tissue culture Rapid propagation MS mdeia Plantlet |
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