| 南天竹组培快繁技术 |
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| 引用本文： | 王春,张俊林,范文锋,王越,方辉.南天竹组培快繁技术[J].林业科技开发,2011,25(2):85-88. |
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| 作者姓名： | 王春 张俊林 范文锋 王越 方辉 |
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| 作者单位： | 浙江森禾种业股份有限公司,杭州,310032 |
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| 基金项目： | 浙江省重大科技专项重点项目 |
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| 摘 要： | 对彩色园林植物南天竹组培快繁技术进行研究,结果表明:MS+1.5 mg/L(质量浓度,后同)BA+0.1mg/L IBA是最佳的增殖配方,增殖系数可达7.3;将3 cm长的嫩茎经壮苗培养和MS+0.25 mg/L BA处理后,转接到1/2MS+0.25 mg/L IBA+0.1 mg/L NAA+0.2 g/L活性炭,且pH为6.5生根培养基中,生根率高达87%以上。在增殖培养基中添加1.0 g/L的50%多菌灵可湿性粉剂能有效地抑制内生菌。
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| 关 键 词： | 南天竹 组培 增殖 生根 |
A study on tissue culture of Nandina domestica |
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| WANG Chun,ZHANG Jun-lin,FAN Wen-feng,WANG Yue,FANG Hui Zhejiang Senhe Seed Co.,Ltd.,Hangzhou ,China.A study on tissue culture of Nandina domestica[J].China Forestry Science and Technology,2011,25(2):85-88. |
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| Authors: | WANG Chun ZHANG Jun-lin FAN Wen-feng WANG Yue FANG Hui Zhejiang Senhe Seed Co Ltd Hangzhou China |
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| Institution: | WANG Chun,ZHANG Jun-lin,FAN Wen-feng,WANG Yue,FANG Hui Zhejiang Senhe Seed Co.,Ltd.,Hangzhou 310012,China |
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| Abstract: | Tissue culture technics of Nandina domestica was studied in this paper.The results showed that the most appropriate proliferation formula was MS+1.5 mg/L BA+0.1 mg/L IBA,its proliferation coefficient was 7.3.When young stems which were cultured in a medium(MS+0.25 mg/L BA) reached 3 cm long,They were transferred into a rooting culture medium 1/2MS+0.25 mg/L IBA+0.1 mg/L NAA+0.2 g/L AC(pH=6.5)]. The rooting rate could reach over 87%.The proliferation medium with 1.0 g/L 50% carbendazol wettable powder added... |
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| Keywords: | Nandina domestica tissue culture proliferation rooting |
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