色木槭芽直接增殖途径的培养条件

为建立色木槭芽直接增殖的诱导培养体系,以色木槭野生树木休眠芽萌发枝条为材料,进行芽增殖的培养条件研究。结果表明,4月份是适宜休眠芽培养的取材时期。嫩茎在2%次氯酸钠浸泡20 min是最佳消毒方案。NAA浓度对休眠芽萌发和嫩茎生长的影响具有显著差异。MS+0.1 mg/L NAA+20 g/L蔗糖(pH5.8)是适合休眠芽萌发和嫩茎伸长的培养基。培养30天时,腋芽萌发率可达63.3%,嫩茎平均高度可达15.9 mm。6-BA对芽直接增殖的促进效果好于KT。不同激素组合中,IBA与6-BA组合对芽增殖和丛生芽生长的效果好于NAA与6-BA组合、NAA与KT组合、IBA与KT组合。MS+0.1 mg/L IBA+1 mg/L 6-BA+20 g/L蔗糖是适合芽增殖和丛生芽生长的培养基。培养30天时,芽增殖率可达90%,增殖倍数可达3.19,且茎芽生长正常。

The tissue culture system for microshoot proliferation through bud organgenesis of Acer mono Maxim

Culture conditions for shoot proliferation from sproutings of dormant axillary buds collected on field trees of Acer mono Maxim were checked for the purpose of establishing a direct shoot proliferation system.The results showed that April was the most suitable periods for collecting dormant buds;the maximum disinfection method for sproutings from dormant buds was souking in 2% sodium hypochlorite(NaClO) for 20 min;NAA concentrations had significant effect on dormant bud sprouting and the growth of the sproutings;MS + 0.1 mg/L NAA+20 g/L sucrose(pH=5.8 before autocalving) was the suitable medium for dormant bud sprouting and the growth of the sproutings;the sprouting rate of axillary buds was 63.3% and the average height of sproutings was 15.9 mm at the 30 days of culture;6-BA was better than KT for direct shoot multiplication;the combination of IBA and 6-BA was better than that of NAA and 6-BA,NAA and KT,IBA and KT for proliferation and growth of the in vitro culture shoots;the best suitable medium for proliferation and growth of the in vitro cultured shoots was MS + 0.1 mg/L IBA +1 mg/L 6-BA + 20 g/L sucrose,the proliferation rate and fold could reach 90% and 3.19 respectively,and the shoots grew well at the 30 days of culture.