| 竹材木质素降解菌的筛选与鉴定* |
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| 引用本文： | 刘剑,李南林.竹材木质素降解菌的筛选与鉴定*[J].广东林业科技,2020,36(1):30-35. |
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| 作者姓名： | 刘剑 李南林 |
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| 作者单位： | 肇庆市林业局森林病虫害防治检疫站 广东肇庆 526040,肇庆市林业局森林病虫害防治检疫站 广东肇庆 526040 |
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| 摘 要： | 竹材木质素的降解一直是制约竹材资源利用的重要影响因素。文章通过对49 份腐烂的竹材样品进行平板划线分离纯化得到74 株真菌，利用PDA- 愈创木酚变色及PDA- 苯胺蓝退色反应，初步筛选出具有木质素降解酶能力的4 株木质素降解菌，通过酶活力测定对初筛得到的4 株木质素降解菌进行复筛，发现FG-22 菌株具有较好木质素降解能力，对FG-22 降解菌株进行形态学和分子生物学鉴定，确定FG-22 为杂色云芝（Trametes versicolor）。
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| 关 键 词： | 木质素降解菌 竹材 筛选 鉴定 |
| 收稿时间： | 2019/12/30 0:00:00 |
| 修稿时间： | 2019/12/30 0:00:00 |
Screening and Identification of Bamboo Lignin Degradation Fungus |
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| LIU Jian and LI Nanlin.Screening and Identification of Bamboo Lignin Degradation Fungus[J].Forestry Science and Technology of Guangdong Province,2020,36(1):30-35. |
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| Authors: | LIU Jian and LI Nanlin |
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| Institution: | Zhaoqing Forestry Bureau Forest Pest Control and Quarantine Station,Zhaoqing,Zhaoqing Forestry Bureau Forest Pest Control and Quarantine Station,Zhaoqing |
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| Abstract: | The degradation of bamboo lignin has always been an important factor influencing the utilization of bamboo resources. Seventy-four fungi were isolated by streaking and purifying 49 samples of rotten bamboo. The lignin-degrading fungi producing lignin-degrading enzyme activity were screened by PDA-guaiacol phenol discoloration and PDA-aniline blue fading reaction. Four strains of lignin-degrading fungi were screened by enzyme activity assay. It was found that FG-22 fungus had better lignin degradation ability, and the FG-22-degrading fungus was identified by morphological identification and molecular biology to determine FG-22 is Trametes versicolor. |
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