马尾松叶表微生物多样性

为发掘对林木生长发育有利的优良微生物资源,并筛选适合叶表微生物的收集方法,以马尾松针叶为试验材料,分别用悬摇法和超声波法收集马尾松叶表微生物,用扩增子高通量测序技术、MUSCLE和Qiime软件研究马尾松叶表微生物的多样性。结果表明:扫描电镜观测结果显示,马尾松针叶表面定殖有大量微生物,包括真菌(菌丝及孢子)和细菌。扩增子高通量测序结果表明,马尾松叶表微生物物种丰富,包含细菌运算分类单位(OTUs)490个,真菌OTUs 1273个。马尾松叶表细菌以未分类的蓝细菌属(unidentified_Cyanobacteria)(36.53%)、未分类的拜叶林克氏菌属(unidentified_Beijerinckia)(28.60%)、鞘氨醇单胞菌属(Sphingomonas)(2.35%)为优势属;叶表真菌以枝孢属(Cladosporium)(2.45%)、拟盘多毛孢属(Pestalotiopsis)(0.92%)、无头孢菌属(Capnobotryella)(0.91%)为优势属。针对叶表细菌多样性的研究表明,悬摇法和超声波法均有较高的物种检出度;在叶表真菌多样性的研究中超声波法优于悬摇法,但超声波法样品间数据变异性较大,测定结果不稳定。

Epiphytic microbial diversity of Pinus massoniana needles

To explore microbial resources that are beneficial for the growth and development of trees,and to evaluate suitable collection methods for leaf surface microorganisms,the needles of Pinus massoniana were collected and microorganisms were disassociated using sonication and rotation methods.Furthermore,the diversity of bacteria and fungi on the leaf surface of P.massoniana was studied using amplicon high-throughput sequencing technology.Using a scanning electron microscope,a large number of microorganisms,including fungi(hyphae and spores)and bacteria colonized on the surface of P.massoniana needles,were detected.The results of high-throughput sequencing showed that there were abundant microbial species on the leaf surface of P.massoniana.A total of 490 bacterial operational taxonomic units(OTUs)and 1273 fungal OTUs were detected.The dominant genera of the leaf surface bacteria were classified in the current study as unidentified_Cyanobacteria(36.53%),unidentified_Beijerinkia(28.60%),and Sphingomonas(2.35%),whereas Cladosporium(2.45%),Pestalotiopsis(0.92%),and Capnobotriella(0.91%)were the dominant genera of fungi found.The study on bacterial diversity on the leaf surface showed that both the sonication and rotation methods had a high degree of species detection,and that the sonication method was superior to the rotation method regarding the identification of fungal diversity.However,the data variability among samples using the sonication method was large,and the determination results were unstable.