广东紫珠抑菌有效部位的筛选

以广东紫珠药材粉末为原料，探讨其不同提取部位的抑菌作用，并筛选其有效部位。对广东紫珠药材用不同极性大小溶剂（石油醚、二氯甲烷、乙酸乙酯、乙醇、70%乙醇、水）回流提取，获得广东紫珠粗提物，用滤纸片法测定其抑菌圈大小（金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌、白色念珠菌），对抑菌活性明显的提取部位进行梯度萃取，并分别测定其抑菌圈大小，得到广东紫珠抑菌有效部位，并测定其有抑菌作用的有效部位的最小抑菌浓度。结果显示广东紫珠70%乙醇提取物对金黄色葡萄球菌、大肠杆菌、奇异变形杆菌、甲型副伤寒沙门氏菌、乙型溶血性链球菌均表现了不同程度的抑菌活性，初步判定为广东紫珠的抑菌活性部位，70%广东紫珠提取物经过萃取后，石油醚萃取物及二氯甲烷萃取物没有抑菌作用，乙酸乙酯萃取物、正丁醇萃取物及剩余的水部分均有不同程度的抑菌作用。70%乙醇广东紫珠提取物通过二氯甲烷萃取除去部分脂溶性杂质，即得到广东紫珠抑菌部位，可以此为基础寻找广东紫珠抑菌活性成分，为后期开发利用提供理论依据。

Screening for bacteriostatic activity of components from Callicarpa kwangtungensis

To investigate and screen the antibacterial activity of different extracts from Callicarpa kwangtungensis, it was extracted by systematic solvents method and separated into six parts: petroleum ether extract, dichloromethane extract, ethyl acetate extract, ethanol extract, 70% ethanol-H2O extract, H2O extract. The vitro antibacterial activity on six strains of these extracts was screened. These six strains were Staphylococcus aureus, Escherichia coli, Proteus mirabilis, Salmonella paratyphi-A , Streptococcus hemolytic-β, Canidia albicans. The site with strong antibacterial activity was gradient extracted. Then, the MIC of the extract with big inhibition zone was determined. Results showed that: The extract of 70% ethanol-H 2O had the best antibacterial activity on five strains. Then, the extracts of ethyl acetate, n-butyl alcohol extract and water had different degree of antibacterial activity. 70% ethanol-H2O extract which was extracted by dichloromethane to remove some lipsoluble impurity was the inhibitory effective sites of C. kwangtungensis. The results provide a basis for developing new bacteriostatic drug.