| 橡胶树内生细菌多样性初探及拮抗菌株的筛选 |
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| 引用本文： | 时涛,彭建华,刘先宝,戴英葵,黄贵修.橡胶树内生细菌多样性初探及拮抗菌株的筛选[J].中国森林病虫,2011,30(2). |
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| 作者姓名： | 时涛 彭建华 刘先宝 戴英葵 黄贵修 |
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| 作者单位： | 中国热带农业科学院环境与植物保护研究所,农业部热带农林有害生物入侵监测与控制重点开放实验室,海南,儋州,571737 |
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| 基金项目： | 公益性行业(农业)科研专项子课题，海南省自然科学基金项目，中国热带农业科学院博士启动经费 |
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| 摘 要： | 经过严格的表面消毒后,从成年橡胶树各组织中分离47份内生细菌.各组织中的内生细菌数量和种类由多到少分别为树根、花序、树皮、叶柄、叶片和果实.以橡胶树多主棒孢病菌Corynespora cossiicola(Berk & Curt.)Wei和炭疽病菌Colletotrichum gloeosporioides Penz.为指示菌,从中筛选出有较好作用的2个菌株,菌落形态观察、生理生化特征鉴定和16S rDNA序列分析表明它们是枯草芽孢杆菌Bacillus subtilis和莫哈韦芽孢杆菌B.mojavensis.
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| 关 键 词： | 橡胶树 内生细菌 拮抗作用 |
Rubber endophytic bacteria population diversity and screening of antagonistic strains |
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| SHI Tao,et al..Rubber endophytic bacteria population diversity and screening of antagonistic strains[J].Forest Pest and Disease,2011,30(2). |
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| Authors: | SHI Tao |
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| Institution: | SHI Tao,et al.(Environment and Plant Protection Institute,CATAS,Key Laboratory of Monitoring and Control of Tropical Agricultural and Forest Invasive Alien Pests,Ministry of Agriculture,Danzhou 571737,China) |
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| Abstract: | Forty seven isolates were separated from different tissus of matured rubber after strict surface sterilization.The amounts and species of endophytic bacteria in rubber demonstrated a decreasing trend in the tissues from root,inflorescence,bark,petiole,lamina and fruit.Corynespora cassiicola and Colletotrichum gloeosporioides isolated from rubber were used as indicative pathogens and two strains with relatively strong antimicrobial effect were screened.The colony figure observation,characteristic of physiolo... |
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| Keywords: | rubber endophytic bacteria antagonism |
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