Glycosylsphingolipids from Euonymus japonicus Thunb

The stem bark of Euonymus japonicus Thunb. led to the isolation of three new glycosylsphingolipids (1–3), 1-O--L-rhamnopyranosyl-(1 → 2)-β-D-glucopyranosyl]-(2S,3R,9E)-2-N-(2R)-hydroxystearoyl]-octadecasphinga-9-ene (euojaposphingoside A, 1), 1-O-β-D-glucopyranosyl-(1 → 2)-β-D-glucopyranosyl]-(2S,3R,4R,11E)-2-N-(2R)-hydroxydocasanoyl]-octadecasphinga-11-ene (euojaposphingoside B, 2), 1-O-β-D-glucopyranosyl]-2′-O-β-D-glucopyranosyl]-(2S,3R,4R,11E)-2-N-(2R)-hydroxytetracosanoyl]-octadecasphinga-11-ene (euojaposphingoside C, 3) along with three known glycosylsphingolipids (4–6), 1-O-β-D-glucopyranosyl]-(2S,3R,9E)-3-hydroxymethyl-2-N-(2R)-hydroxynonacosanoyl)-tridecasphinga-9-ene (4), 1-O-β-D-glucopyranosyl]-(2S,3R,9E,12E)-2-N-(2R)-hydroxytetracosanoyl] octadecasphinga-9,12–diene (5), 1-O-β-D-glucopyranosyl]-(2S,3R,5R,9E)-2-N-tridecanoyl] nonacosasphinga-9-ene (6), lupeol (7), stigmasterol (8), sitosterol (β and α) (9,10) and β-carotene (11). The structure of all the compounds was achieved by spectroscopic and chemical data analysis. The antiplasmodial, antileismanial and cytotoxic activity of all compounds was tested.