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| 白玉兰离体培养和快速繁殖 | |
| 引用本文： | 褚建民,周凌娟,王阳,王东启,商政义.白玉兰离体培养和快速繁殖[J].防护林科技,2002(4):29-31. |
| 作者姓名： | 褚建民 周凌娟 王阳 王东启 商政义 |
| 作者单位： | 1. 辽宁省高速公路管理局昌图处,辽宁,昌图,112500 2. 沈阳农业大学林学系 3. 中国医科大学 4. 盘锦市绿化处 5. 辽河油田茨榆坨采油厂 |
| 摘 要： | 试验建立了白玉兰离体繁殖体系。种子为外植体 ,初代培养基为 :B5 +BA0 .5mg/L +NAA0 .0 5mg/L + 30g/L糖 +琼脂 6g/L ,萌发率 85 %。继代培养基为 :B5 +BA1.5mg/L +NAA0 .15mg/L + 30g/glucose/sucrose + 6g/Lagar+Vco0 .5g/L ,月增殖率可达 5 .0± 0 .1。生根培养为 :1/2B5+NAA0 .5mg/L +AC1.0mg/L +VB2 0mg/L。以试管苗茎段、根段做外植体 ,培养基B5 +BA2mg/L+ 2 .4 -D0 .5mg/L +VC0 .5g/L ,可获得少量愈伤组织 ,继代存活率仅为 30 %。没有再分化成苗 |
| 关 键 词： | 白玉兰 离体培养 快速繁殖 |
| 文章编号： | 1005-5215(2002)04-0029-03 |
| 修稿时间： | 2002年7月31日 |
IN VITRO CULTURE AND RAPID PROPAGATION OF MAGNOLIA DENUDATA |
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| CHU Jian min ,ZHOU Ling juan ,WANG Yang ,WANG Dong qi ,SHANG Zheng yi.IN VITRO CULTURE AND RAPID PROPAGATION OF MAGNOLIA DENUDATA[J].Protection Forest Science and Technology,2002(4):29-31. | |
| Authors: | CHU Jian min ZHOU Ling juan WANG Yang WANG Dong qi SHANG Zheng yi |
| Institution: | CHU Jian min 1,ZHOU Ling juan 2,WANG Yang 3,WANG Dong qi 4,SHANG Zheng yi 5 |
| Abstract: | The system of tissue culture and rapid propagation of Magnolia denudata was established. Explants are seed. The most suitable medium is B5 or SH + BA0.5mg/L+ NAA0.05mg/L+30 g/L sugar +6g/L agar, with a germination rate of 85%. The best medium for proliferating was B5+BA1.5mg/L+NAA0.15mg/L+glucose or sucrose 30g/L +6g/L agar +Vco0.5g/L, with a monthly proliferating rate of 5.0±0.1. The rooting medium is 1/2B5+NAA0.5mg/L +AC1.0mg/L+VB20mg/L. Callus could be induced when the stem and root segments are cultured on the medium of B5+BA2mg/L+2.4 - D0.5mg/L+VC0.5g/L, but the succeeding survival rate is only 30% and the callus was not induced to plant. |
| Keywords: | Magnolia denudata In vitro culture Rapid propagation |
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