| 银中杨茎段离体培养再生植株研究 |
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| 引用本文： | 刘红梅.银中杨茎段离体培养再生植株研究[J].防护林科技,2011(1):36-38. |
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| 作者姓名： | 刘红梅 |
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| 作者单位： | 辽宁省阜新市林业局,辽宁,阜新,123000 |
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| 摘 要： | 以银中杨茎段为外植体,建立组织培养系统.试验结果表明,MS为最适基本培养基;各激素对诱导不定芽的作用大小为TDZ＞6-BA＞KT,诱导不定芽的最佳培养基为MS+0.1 mg·L-1TDZ+蔗糖20 g·L-1+琼脂0.7%;诱导不定芽再生的最佳光质为白光或黄光;生根培养基以1/2MS+0.5 mg·L-1IBA+蔗糖2...
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| 关 键 词： | 银中杨 茎段 组织培养 |
Stem Segments of Populus alba × P.berolinensis Plant Regeneration in Vitro |
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| LIU Hong-mei.Stem Segments of Populus alba × P.berolinensis Plant Regeneration in Vitro[J].Protection Forest Science and Technology,2011(1):36-38. |
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| Authors: | LIU Hong-mei |
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| Institution: | LIU Hong-mei (Forestry Bureau of Fuxin,Liaoning Province,Fuxin 123000,China) |
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| Abstract: | Taking Populus alba ×P.berolinensis stems as explants,tissue culture system was established.Result shows that: MS is the optimal basic medium;various hormones on the size of the role of adventitious buds is TDZ> 6-BA> KT;the optimal medium for induction of adventitious buds is MS + 0.1 mg·L-1 TDZ + sucrose 20 g·L-1+ agar 0.7%;induced shoot regeneration of the optimal quality is white light or yellow light;the optimal rooting medium is 1/2MS + 0.5 mg ·L-1 IBA + sucrose 20 g·L-1 + agar 0.7%;the rooting rate reaches 100%;the average root number are 3.9. |
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| Keywords: | Populus alba ×P berolinensis stem tissue culture |
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