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| 大花天竺葵组织培养初探 | |
| 引用本文： | 程建军,曹爱珍,陈瑶,杨刚.大花天竺葵组织培养初探[J].四川林业科技,2011,32(1):130-132,125. |
| 作者姓名： | 程建军 曹爱珍 陈瑶 杨刚 |
| 作者单位： | 1. 四川省仪陇县林业局,四川,仪陇,637600 2. 四川省仪陇县农业局,四川,仪陇,637600 3. 四川农业大学林学园艺学院,四川,雅安,62501 |
| 摘 要： | 以‘亚里士多德’系列的大花天竺葵新品种的叶、叶柄和带芽茎段为外植体,进行了不定芽、愈伤组织的诱导及芽萌发的研究。结果表明,大花天竺葵叶片诱导不定芽分化的最适培养基为MS＋0.5 mg·L^-1NAA＋3.0·L^-16-BA,诱导率75.61%,平均不定芽个数4.98;叶柄诱导愈伤组织的最适培养基为MS＋0.3·L^-1NAA＋1.0·L^-16-BA,诱导率47.2%;带芽茎段诱导不定芽的最适培养基为MS＋0.5·L^-1NAA＋1.0·L^-16-BA,诱导率81.27%。 |
| 关 键 词： | 大花天竺葵 带芽茎段 组织培养 |
A Primary Study of the Tissue Culture of Pelargonium domesticum |
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| CHENG Jian-jun,CAO Ai-zhen,CHEN Yao,YANG Gang.A Primary Study of the Tissue Culture of Pelargonium domesticum[J].Journal of Sichuan Forestry Science and Technology,2011,32(1):130-132,125. | |
| Authors: | CHENG Jian-jun CAO Ai-zhen CHEN Yao YANG Gang |
| Institution: | 1.Forestry Bureau of Yilong County,Yilong 637600,Sichuan;2.Agriculture Bureau of Yilong County,Yilong 637600,Sichuan;3.Sichuan Agricultural University,Yaan 625014,Sichuan) |
| Abstract: | leaves,petioles and stems with buds of Aristotle series Pelargonium domesticum Bailey were used as explants for researches into the induction of adventitious bud and callus formation.The results showed that the optimum medium for adventitious bud induction of leaves was MS＋ 0.5 mg·L^-1 NAA ＋3.0 mg·L^-1 6-BA.The induction rate was 75.61%,and each explant could produce 4.98 adventitious buds.The appropriate medium for callus formation of petioles was MS＋0.3 mg·L^-1 NAA ＋1.0 mg·L^-1 6-BA,and the callus induction rate was 47.2%.The optimum medium for adventitious bud induction of stems with buds was MS＋0.5 mg·L^-1 NAA ＋1.0 mg·L^-1 6-BA,and the induction rate was 81.27%. |
| Keywords: | Pelargonium domesticum Bailey stem with buds primary culture |
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