姜黄素纳米混悬剂的制备及其表征

为了提高姜黄素的水溶性,采用高压均质法制备姜黄素纳米混悬剂,优化了姜黄素纳米混悬剂的制备工艺。利用红外光谱(IR)、紫外光谱(UV)、X射线衍射(XRD)、透射电镜(TEM)和高效液相色谱(HPLC)对姜黄素纳米混悬剂进行了理化性质和化学结构的分析。结果表明:当均质压力100 MPa,循环次数30次时,姜黄素纳米混悬剂的平均粒径为(171.00±8.56)nm,一致性系数为(0.69±0.05);IR、UV、XRD、TEM和HPLC分析表明,姜黄素纳米混悬剂冻干粉的化学结构未见明显改变,姜黄素纳米粒子为不规则的球状晶体,结晶度显著降低。姜黄素纳米混悬剂冻干粉在水中的溶解度达62.85 mg/L,比姜黄素原粉提高了90.48倍;姜黄素纳米混悬剂冻干粉体外溶出速率也明显提高,120 min时溶出度已达80%。研究表明高压均质法适用于姜黄素纳米混悬剂的制备。

Preparation and Characterization of Curcumin Nanosuspensions

In order to improve the water solubility of curcumin, curcumin nanosuspensions were prepared by high pressure homogenization technology, and the preparation process was optimized. The physicochemical properties and chemical structure of curcumin nanosuspensions were investigated by UV spectrometry, infrared spectrometry ( IR ) , X-ray diffractometry ( XRD ) , transmission electron microscopy ( TEM) and high performance liquid chromatography ( HPLC) . The results showed that the mean particle size of curcumin nanosuspension was (171. 00 ± 8. 56) nm, and the PI was (0. 69 ± 0. 05) under optimal conditions of 100 MPa and 30 cycles. UV, IR, XRD,TEM and HPLC results showed that the chemical structures of curcumin nanosuspensions were not obviously changed, the curcumin nanoparticles were irregular spherical form, and the crystallinity decreased. The water solubilities of curcumin nanopowder were 62. 85 mg/L enhanced by 90. 48 times compared with curcumin powder. The dissolution velocities were significantly improved, and the dissolution rate reached up to 80% at the time of 120 min. The research indicated that high pressure homogenization technology could be successfully used to prepare curcumin nanosuspensions.