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库尔勒香梨砧木组织培养
引用本文:曾斌,李疆,张孝霖,张强,史兰花,郭玉成.库尔勒香梨砧木组织培养[J].经济林研究,2006,24(2):41-43.
作者姓名:曾斌  李疆  张孝霖  张强  史兰花  郭玉成
作者单位:1. 新疆农业大学园艺学院,校级重点植物组织培养实验室,新疆,乌鲁木齐,830052
2. 库尔勒香梨股份有限公司,新疆,库尔勒,841000
摘    要:试验选用库尔勒香梨矮化中间砧S系、F系中的品种嫩茎尖作为试验材料进行了离体增殖研究,结果表明,不同种类的基本培养基及不同浓度配比的植物生长调节剂对试管苗增殖的效果不同。M S培养基较A S、B5培养基更有利于矮化砧S系、F系茎的增殖,中等浓度的细胞分裂素(BA)与较低浓度的生长素(IBA)、赤霉素(GA3)配合使用效果更理想。综合茎质量、茎数量等指标认为:M S BA 1.0 m g/L IBA 0.05 m g/L GA31.0 m g/L是矮化砧S系、F系试管苗茎增殖的最佳培养基。

关 键 词:库尔勒香梨  矮化中间砧S系、F系  离体增殖
文章编号:1003-8981(2006)02-0041-03
收稿时间:2006-03-08
修稿时间:2006年3月8日

Study on Proliferation in Vitro of Dwarfing Interstock S and F series in Kuerle Fragrant Pear
ZENG Bin,LI Jiang,ZHANG Xiao-Lin,ZHANG Qiang,SHAN Lan-Hua,GUO Yu-Cheng.Study on Proliferation in Vitro of Dwarfing Interstock S and F series in Kuerle Fragrant Pear[J].Economic Forest Researches,2006,24(2):41-43.
Authors:ZENG Bin  LI Jiang  ZHANG Xiao-Lin  ZHANG Qiang  SHAN Lan-Hua  GUO Yu-Cheng
Institution:1. Key Laboratory of Plant Tissue Culture, College of Horticultural, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China; 2. Kuerle Fragrant pear Co. Ltd. , Kuerle 841001, Xinjiang, China
Abstract:Taken dwarfing interstock S and F series in Kuerle fragrant pear as materials,tender stem tips were cultured in vitro.The results showed that the effects of kinds and concentrations of plant growth regular on proliferation in vitro were different.MS medium was more suitable for proliferation of stems than AS and B5 media,otherwise,combination of BA of medium concentration,GA3and IBA of lower concentration could have an ideal result.MS medium supplemented with BA(1.0 mg/L),IBA(0.05 mg/L) and GA3(1.0 mg/L) was optimum for proliferation and growth of shoots,comprehensively considering the indexes,for example,quality and quantity of stems.
Keywords:Kuerle fragrant pear  dwarfing interstock S and F series  proliferation in vitro
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