也门铁工厂化育苗技术

以也门铁嫩茎段为材料,开展了旨在用于工厂化育苗的组织培养技术研究。研究结果表明,外植体在M S 3 m g/L BA 0.2~0.5 m g/L IAA培养基上,在腋芽萌发生长同时也启动其基部周围的分化;转入M S 2~3 m g/L BA 0.2~0.3 m g/L IAA的培养基上,每外植体能平均分化出1 mm以上的不定芽5~6个,不定芽在M S 0.5 m g/L BA 0.05 m g/L IAA培养基上进行增殖后,将其中高度大于1.2 cm的不定芽转入M S 0.05 m g/L BA 0.05 m g/L IAA 0.5%活性炭培养基上进行壮苗,再转入1/2M S 0.5m g/L IBA培养基生根,最后经练苗并移栽至网室内生长,获得供大田栽培的苗。保证适度的增殖率(约400%)、获得较粗壮的芽体、控制变异和简化培养条件是也门铁大规模组织培养育苗的技术关键。

Techniques of Industrialized Seedling Culture in Dracaena fragrans

Techniques of issue culture in Dracaena fragrans were researched,taken tender stem section as materials,which will be used for industrialized seedling culture.The results showed that cell differentiation was induced at the base of axillary buds while bourgeoning and growing in MS media supplemented with BA 3 mg/L and IAA 0.2-0.5 mg/L;there were 5-6 adventitious buds over 1 mm long per explant differentiated in MS media supplemented with BA 2-3 mg/L and IAA 0.2-0.3 mg/L;after axillary buds proliferated in MS media supplemented with BA 0.5 mg/L and IAA 0.05 mg/L,the buds over 1.2 cm long were transplanted in MS media supplemented with BA 0.05 mg/L,IAA 0.05 mg/L and 0.5% actived carbon,and then in 1/2 MS media supplemented with BA 0.5 mg/L to induce rooting;seedling were transplanted in the soil after hardening.The key techniques of industrialized seedling culture were moderate rate(400%),stronger buds,variation control and culture condition simplification.