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| 苹果枣组培脱毒与快繁技术 | |
| 引用本文： | 袁德义,胡孝义,赵思东.苹果枣组培脱毒与快繁技术[J].经济林研究,2004,22(1):35-36. |
| 作者姓名： | 袁德义 胡孝义 赵思东 |
| 作者单位： | 1. 湖北省襄樊市农业委员会,湖北,襄樊,441021 2. 湖北省林木种苗管理站,湖北,武昌,430079 3. 中南林学院资源与环境学院,湖南,株洲,412006 |
| 摘 要： | 采用热处理与组织培养技术相结合培育苹果枣苗．能有效脱除枣疯病原MLO。对苹果枣组培脱毒与快繁技术的研究结果表明，适宜的诱导培养基为MS BA1．0mg／L KT0．5mg／L 3％蔗糖，继代培养基为MS BA1．5mg／L IBA0．5mg／L 3蔗糖，生根培养基为MS IBA1．0mg／L IAA0．5mg／L 3％蔗糖．试管苗无土移栽成活率为96％。 |
| 关 键 词： | 苹果枣 组织培养 脱毒技术 快繁技术 |
| 文章编号： | 1003-8981(2004)01-0035-02 |
| 修稿时间： | 2003年9月14日 |
Virus Elution in Tissue Culture and Fast Propagation in Jujube Pingguozao |
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| YUAN De-Yi,HU Xiao-Yi,ZHAO Si-Dong.Virus Elution in Tissue Culture and Fast Propagation in Jujube Pingguozao[J].Economic Forest Researches,2004,22(1):35-36. | |
| Authors: | YUAN De-Yi HU Xiao-Yi ZHAO Si-Dong |
| Institution: | YUAN De-Yi~1,HU Xiao-Yi~2,ZHAO Si-Dong~3 |
| Abstract: | Jujube witches broom is one of the diseases that severely affect the production in jujube (Ziziphus jujuba). The combination of heat treatment and tissue culture effectively eluted the MLO pathogen in the seedlings of pingguozao, one of the fine jujube varieties recently released. Studies indicated that the optimal inducing medium for virus elution through tissue culture and for fast propagation is MS+BA 1.0 mg/L+KT 0.5 mg/L+3% sucrose; the ideal medium for secondary culture is MS+BA 1.5 mg/L+IBA 0.5 mg/L+3%sucrose; and the best rooting medium tested was MS+IBA 1.0 mg/L+IAA 0.5 mg/L+3% sucrose. The survival percentage of the transplanted soil-free test-tube plantlets reached 96 percent. |
| Keywords: | Pingguozao tissue culture fast propagation technique |
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