| 棕榈藤基因组DNA提取及RAPD反应条件探索 |
| |
| 引用本文： | 李发根,杨华,尹光天,许煌灿,甘四明.棕榈藤基因组DNA提取及RAPD反应条件探索[J].林业科学研究,2004,17(6):824-828. |
| |
| 作者姓名： | 李发根 杨华 尹光天 许煌灿 甘四明 |
| |
| 作者单位： | 中国林业科学研究院热带林业研究所,广东,广州,510520 |
| |
| 基金项目： | 国家科技攻关计划(2001BA506b04 01) |
| |
| 摘 要： | 棕榈藤(rattan)是棕榈科(Palmae Juss．)藤本植物，属棕榈科省藤亚科(Calamoideae)省藤族(Calameae)植物，包括13属600余种，主要分布于热带地区。棕榈藤是热带森林宝库中的多用途植物资源，具有很高的经济价值和开发前景，是仅次于木材和竹材的重要林产品，其中黄藤(Daemonorops margaritae(Hance)Seccari)和单叶省藤(Calamus simplicifolius Wei)是我国大面积栽培的2个重要藤种。
|
| 关 键 词： | 棕榈藤 基因组 DNA 提取 RAPD 反应条件 |
| 文章编号： | 1001-1498(2004)06-0824-05 |
| 收稿时间： | 2003/12/8 0:00:00 |
Genomic DNA Extraction and RAPD Protocols for Rattan |
| |
| LI Fa-gen,YANG Hu,YIN Guang-tian,XU Huang-Can and GAN Si-ming.Genomic DNA Extraction and RAPD Protocols for Rattan[J].Forest Research,2004,17(6):824-828. |
| |
| Authors: | LI Fa-gen YANG Hu YIN Guang-tian XU Huang-Can and GAN Si-ming |
| |
| Institution: | Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China |
| |
| Abstract: | Genomic DNA was successfully extracted from leaf tissues of two rattan species,Calamus simplificifolius and Daemonorops margaritae,using a modified CTAB method.This method could remove phenolic compounds,polysaccharides and proteins,and the quality and quantity of DNA extracted were reliably characterized.In addition,the reaction composition and amplification program for RAPD analysis were developed with GeneAmp PCR System 2700 (Applied Biosystem Co.).This study provided a technological basis for future molecular studies in rattan and the related species. |
| |
| Keywords: | rattan genomic DNA extraction CTAB RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
| 点击此处可从《林业科学研究》下载免费的PDF全文 |
|
