苏芸金芽孢杆菌孢子囊超微结构的研究

用电子显微镜观察了分属于苏芸金芽孢杆菌(Bacillus thuringiensis)23个 H-血清型的30个亚种和3株新分离菌株孢子囊超薄切片的超微结构。结果表明苏芸金杆菌30个亚种的芽孢和伴孢晶体在孢子囊中形成的部位、形状及数量有所不同。大部分亚种的伴孢晶体位于孢子囊的一端,以单个晶体存在于孢外膜之外,只有幕虫亚种(Subsp.finitimus)的伴孢晶体位于孢外膜之内。在孢子囊中晶体的形状分别为菱形、方形、圆形、不规则形。其数量大多数为一个。从不同森林土壤中分离到的87052、85020、85040三株菌,其中87052菌株伴孢晶体为圆形,与已知圆形伴孢晶体的亚种形态特征相似,其它两株菌在形态特征上与已知 30个亚种在孢子囊的超微结构上有着一定的差异。

STUDIES ON THE ULTRAMICRO STRUCTURE OF BACILLUS THURINGIENSIS SPORANGIUM

The thin-sections of ultramicro structure of Bacillus thuringiensis belonging to 30 subspecies of 23 H-serotypes and 3 straines isolated from the soil were examined by electron microscopy. The result showed that the forming position, the form and the quantity of spore and crystal of the 30 subspecies in sporangium vary from each other. In most of the species, the spore is located on one side of the sporangium and outside of exosporium. The crystal of Bacillus thuringiensis subspecies finitimus is located inside of exosporium. The form of crystal in the sporangium of various subspecies varyes greatly, which is usually dipyramidal, spheroidal, cuboidal or irregular in shape and most of them were single one.In the 3 strains isolated from soil, 87052 strain is similar to H23 (Subsp. japonensis), 85020 and 85040 strains were different from the 30 subspecies.