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红树林根际解磷菌分离、培养及解磷能力的研究
引用本文:万璐,康丽华,廖宝文,马海宾,江业根.红树林根际解磷菌分离、培养及解磷能力的研究[J].林业科学研究,2004,17(1):89-94.
作者姓名:万璐  康丽华  廖宝文  马海宾  江业根
作者单位:中国林业科学研究院热带林业研究所,广东,广州,510520
基金项目:广东省技术厅国际合作项目"红树林PGPB菌研究(2KB0682W)
摘    要:本实验从红树林根际分离出不同种类的解磷细菌,经过筛选获得一株解磷能力较强的菌株ZB0211,在纯培养的条件下,对这株解磷菌进行最适培养条件试验。结果表明:碳源以10g·kg-1的蔗糖最佳,氮源以1g·kg-1NH4Cl最好,10g·kg-1NaCl浓度为最适生长浓度,最适初始pH值为7 5。同时进行的解磷能力实验也表明pH值、菌数与解磷能力这三者之间存在一定的相关性。

关 键 词:红树林  根际解磷菌  分离  培养  解磷强度  磷元素
文章编号:1001-1498(2004)01-0089-06
收稿时间:2003/6/15 0:00:00

Mangrove PSB:Isolation,Culture and Phosphate-dissolving Ability
WAN Lu,KANG Li-hu,LIAO Bao-wen,MA Hai-bing and JIANG Ye-gen.Mangrove PSB:Isolation,Culture and Phosphate-dissolving Ability[J].Forest Research,2004,17(1):89-94.
Authors:WAN Lu  KANG Li-hu  LIAO Bao-wen  MA Hai-bing and JIANG Ye-gen
Institution:Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China;Research Institute of Tropical Forestry, CAF, Guangzhou510520, Guangdong, China
Abstract:Ten phosphate solubilizing bacterial(PSB) strains were isolated from the mangrove rhizospere.The culture condition of the selected most effective strain ZB0211 was examined from 4 aspects.The experimental results showed that the optimal carbon and nitrogen sources were respectively sucrose (10 g·kg-1) and NH4Cl ((1 g·kg-1)),the optimal concertration of NaCl was 1%,and optimal pH 7.5.The potential relationship among pH level,bacterial rowth and phosphate-dissolving ability was also discussed.
Keywords:mangrove  PSB  isolation  culture condition  phosphate-dissolving ability
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