| 乐昌含笑种群遗传多样性的研究 |
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| 引用本文： | 姜景民,滕花景,袁金玲,栾启福,谭梓峰.乐昌含笑种群遗传多样性的研究[J].林业科学研究,2005,18(2):109-113. |
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| 作者姓名： | 姜景民 滕花景 袁金玲 栾启福 谭梓峰 |
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| 作者单位： | 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400 |
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| 基金项目： | 科技部基础项目"亚热带珍稀树种种质资源收集、保存及编目"(2002DEA1000203)课题,浙江省林木良种技术支撑项目"浙江省阔叶绿化树种选育和快繁技术研究" |
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| 摘 要： | 基于随机扩增多态DNA(RAPD)方法分析了珍稀木兰科植物乐昌含笑6个种群的遗传多样性及分化程度。19条随机引物扩增出111个可分析位点,多态位点百分比为81．98％,经POPGENE分析发现,乐昌含笑的Nei’s基因多样度(Hc)为0．3255,Shannon表型指数(I)为0．4751,与其它植物比较具有较高水平的遗传多样性。6个乐昌含笑群体间基因分化系数(Gst)值为0．2226。群体内的遗传变异大于群体问的遗传变异。按照遗传距离将乐昌含笑6个群体划分为两类：第1类有广西三江、湖南宜章、湖南双牌和湖南资兴的群体,第2类包括江西官山和湖南醴陵的群体;研究表明：第2类种群的遗传多样性指数高于第1类种群的遗传多样性指数。
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| 关 键 词： | 乐昌含笑 RAPD 遗传多样性 群体分化 |
| 文章编号： | 1001-1498(2005)02-0109-05 |
| 收稿时间： | 7/1/2004 12:00:00 AM |
Genetic Diversity of Michelia chapensis Dandy Populations |
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| JIANG Jing-min,TENG Hua-jing,YUAN Jin-ling,LUAN Qi-fu and TAN Zi-feng.Genetic Diversity of Michelia chapensis Dandy Populations[J].Forest Research,2005,18(2):109-113. |
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| Authors: | JIANG Jing-min TENG Hua-jing YUAN Jin-ling LUAN Qi-fu and TAN Zi-feng |
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| Institution: | Research Institute of Subtropical Forestry, CAF, Fuyang311400, Zhejiang, China;Research Institute of Subtropical Forestry, CAF, Fuyang311400, Zhejiang, China;Research Institute of Subtropical Forestry, CAF, Fuyang311400, Zhejiang, China;Research Institute of Subtropical Forestry, CAF, Fuyang311400, Zhejiang, China;Research Institute of Subtropical Forestry, CAF, Fuyang311400, Zhejiang, China |
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| Abstract: | Michelia chapensis ,belonging to Michelia genus in the family Magnoliaceae, is a elite species for timber production and for landscaping. In this paper,the genetic diversity were investigated using RAPD technique.The total DNA were extracted following a modification of CTAB method with some adjustments. A total of 144 individuals representing 6 natural population of M. chapensis were surveyed using 19 RAPD primers.At species level,the percentage of polymorphic bands was 81.98%,Nei's gene diversity was 0.325 5 and Shannon index was 0.475 1.AMOVA showed that 29.96% of the genetic diversity resulted from differentiation among populations. The coefficient of gene differentiation(Gst)generated by POPGENE32 was 0.222 6. The 6 natural populations of M. chapensis might be divided into 2 groups used UPGMA cluster system,and the genetic diversity of second group was higher than that of the first. |
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| Keywords: | Michelia chapensis RAPD genetic diversity population differentiation |
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