红花石蒜遗传多样性的ISSR分析

利用ISSR分子标记技术对14个居群的红花石蒜进行遗传多样性研究,结果表明:POPGEN32分析显示红花石蒜物种的遗传多样性很高,多态位点百分率为92.31%,Shannon指数(H)为0.459 7,Ne i指数(I)为0.302 5;居群水平的遗传多样性较低,多态位点百分率平均为49.65%,Shannon指数(H)平均为0.262 0,Ne i指数(I)平均为0.176 3;居群间的遗传分化系数(Gst)为0.503 5,基因流(Nm)为0.698 3。AMOVA分子变异分析显示:居群间遗传分化程度高,46.12%的变异发生在居群内,53.88%的变异发生于居群间。生境的片段化使居群间的基因流受阻,可能是居群间高遗传分化和居群水平低遗传多样性的主要原因。

Analysis of Genetic Diversity in Lycoris radiata Using ISSRMarker

The genetic diversity of 14 Lycoris radiata populations was analyzed by ISSR markers.The results of POPGEN32 analysis showed that at species level,the percentage of polymorphics was 92.31%,Shannon's index(H) was 0.459 7,and Nei's gene diversity(I) was 0.302 5,indicating a high level of genetic diversity.But at population level,they were 49.65%,0.262 0 and 0.176 3,respectively,suggesting a low level of genetic diversity.And the gene differentiation coefficient(Gst) and gene flow(Nm) among the populations were 0.503 5 and 0.698 3,respectively.The analysis of molecular variance(AMOVA) demonstrated that there was a relatively high level genetic variation(46.12%) among the populations,and there was relatively low genetic variation(53.88%) within the populations.The high genetic differentiation among populations and the low genetic diversity within populations could be attributed to the habitat fragmentation and the limited gene flow among populations.