酯酶在根结线虫分类上应用的研究

应用聚丙烯酰胺凝胶电泳技术,对采自我国14个省市的5种根结线虫(Meloid-ogyne spp.),共47个样本作了正向极酯酶的分析;并研究了一些可能影响测定的因素。结果表明,根结线虫种间的酯酶谱存在明显而稳定的特异性,在种内也是稳定的。对接种寄主、样本来源、生理小种、室内培养与田间自然寄生、营养等不同条件下的对比分析表明,它们均不影响种的特征性酶谱。因此将酯酶作为根结线虫分类的辅助手段是有意义的。但线虫的发育阶段不同,酯酶谱有差异。采用产卵期的雌成虫进行分析则简便、可靠。

STUDY ON THE APPLICATION OF ESTERASE PHENOTYPES IN THE TAXONOMY OF MELOIDOGYNE SPP.

The author used polyacrylamide gel electrophoresis method to compare the esterase isozymes of 47 isolates of 5 Meloidogyne spp. originated from 14 provinces (cities) in China. Some factors which maybe affect enzyme phenotypes were also studied. The result showed that the esterase phenotypes of egg-laying female were evident, stable and species specific. It was stable intro species too. Comparison of studies on inoculated plants, origin of isolates, physiological races, culture in greenhouse and from plants inffected naturally, different nutritions etc. showed that all of them didn't affect the species specific phenotypes. So the esterase phenotypes could be used as a taxonomic criteria.Since the phenotypes were not the same in different developmental stages for the same species, it was suggested that use of egg-laying females for identification is simple and reliable.