基于SSR标记的油橄榄主要栽培品种子代父本分析

目的]对我国甘肃武都油橄榄品种园主要栽培品种子代进行父本分析,鉴定真实父本,探讨品种亲和性,为品种园授粉品种的选择提供科学依据。方法]以武都油橄榄品种园114份种质为候选父本,基于14对SSR荧光标记,利用Cervus 3.0软件检测亲本分析相关参数,对‘城固32’、‘豆果’、‘贺吉布兰克’和‘皮瓜尔’4个主要栽培品种子代进行真实父本鉴定。结果]14个SSR位点多态性高,累积排除概率随位点数增加而增大。对于单亲已知类非父排除概率(NE-2P),14个位点的累积排除概率高达0.999。通过比较子代、母本和候选父本的基因型,计算出每个候选父本的似然对数比,即LOD值,对油橄榄4个品种部分子代真实父本进行鉴定,其中‘城固32’和‘皮瓜尔’子代的主要父本为‘豆果’,‘豆果’和‘贺吉布兰克’子代的主要父本为‘小苹果’。结论]所选高多态性和高累积排除概率的SSR位点适用于对油橄榄品种子代进行父本分析,为4个品种部分子代鉴定出真实父本,其中仅‘城固32’具有一定的自交亲和性,这为品种建园时授粉品种的选择和配置提供重要依据。

Paternity Analysis of Main Olive Cultivars Progenies Based on SSR Markers

Objective]Paternity analysis of main olive cultivars progenies in Wudu Olive Orchard from Gansu province was carried out to identify the true paternal parent,explore the compatibility,and provide references for the selection of pollination cultivars in the orchard.Method]114 germplasm accessions from Wudu Olive Orchard were used as candidate paternal parents.Based on 14 pairs of fluorescence-labelled SSR markers,Cervus software was used to detect the parameters.True paternal parent identification of the four cultivars progenies(‘Chenggu 32’,‘Arbequina’,‘Hojiblanca’and‘Picual’)was performed.Result]The 14 SSR loci were highly polymorphic and the cumulative exclusion probability increased with the number of loci.For the single parent known type NE-2P,the cumulative exclusion probability of the 14 loci was as high as 0.999.By comparing the genotypes of the progeny,the female parent and the candidate paternal parent,the LOD(log of likelihood ratio)values of each candidate paternal parent were calculated,and the identification of the true paternal parents of the partial progenies of the four cultivars was completed.The main paternal parent of the‘Chenggu 32’and‘Picual’progenies was‘Arbequina’,while the main paternal parent of the‘Arbequina’and‘Hojiblanca’progenies was‘Manzanilla’.Conclusion]The SSR loci with characteristics of high polymorphism and high cumulative exclusion probability selected in the study are suitable for paternity analysis of olive cultivars progenies.The true paternal parents were identified for some of the four cultivars progenies and only‘Chenggu 32’among the four cultivars shows a certain self-compatibility,which provide important basis for the selection and configuration of pollination cultivars in the future orchard establishment.