大花蕙兰(Cymbidium hybridum )试管苗基部切块诱导类原球茎研究

以大花蕙兰试管苗基部切块为外植体,以MS培养基为基本培养基,培养诱导类原球茎(PLB),研究不同激素配比以及外植体来源基因型对PLB诱导的影响.低水平的2,4-D和较高水平的6-BA对于试管苗基部切块诱导PLB具有显著的促进作用.当2,4-D浓度高于0.60 mg·L-1时,PLB诱导受到显著抑制.不同基因型有不同的最佳激素组合.NAA对特定基因型诱导PLB有效.以试管苗基部切块组织培养方式诱导大花蕙兰PLB是可行的,为转基因研究提供了植株再生技术基础.

Micropropagation of Cym bidium hybridum by Inducementof PLB from Cuted Ba se of in vitro Plantlets

PLB ( p rotocorm-like body ) is successfully induced from the cuted tissue of p lantlet2base of Cym bid iumhybridum in vitro, in a simulative manner like microtuber. The effects of different concentrations of 2, 4-D, 6-BA,and NAA on the PLB formation of C. hybridum were investigated in different level of concentration. The resultsshowed that the effects of 2, 4-D and 6-BA on the frequency of PLB formation of C. hybridum differed extremely significantly. 2, 4-D on lower levelwas a important stimulative to induce PLB, but the inducing of PLB would be restrained when the concentration of 2, 4-D was above 0. 60 mg·L^-1. The frequency of PLB formation increased basically with the increase of concentrations of 6-BA. The frequency of PLB formation was also related to the genotype,NAA was a important stimulative factor for some special genotype of C. hybridum. The experiment strategy to inducing PLB in simulative manner of bulb is feasible.