思茅松毛虫3个地理种群性信息素成分差异分析

目的]分析思茅松毛虫雌成虫不同地理种群间性信息素成分、含量和比例差异,比较雄成虫触角敏感性和不同配比诱芯诱捕效果差异,开发不同地域有针对性的思茅松毛虫种群监测方法和诱捕防控技术。方法]运用气相色谱(GC)分析思茅松毛虫湖南、云南和江西地理种群雌虫性信息素腺体提取物,利用触角电位仪(EAG)测定思茅松毛虫各地理种群雄蛾触角对性信息素标准品及其不同配比组分的电生理敏感性,然后进行林间生物测定,比较不同配比的性信息素成分诱蛾效果。结果]思茅松毛虫性信息素腺体中含有顺5,反7-十二碳二烯乙酸酯(Z5,E7-12:OAc)、顺5,反7-十二碳二烯醇(Z5,E7-12:OH)和顺5-十二碳烯乙酸酯(Z5-12:OAc)3种成分,3个地理种群间这3种腺体成分含量和比例存在微小差异。3种成分以100:10:25或者100:10:10的比例配比,不同地理种群间均具有很好的触角电位活性和林间诱蛾活性。结论]思茅松毛虫性信息素组分还没有发生明显的种下分化现象,3个地理种群间性信息素含量和比例的细微差异暗示着与地域差异、寄主植物差异和人为化学防控干扰等因素相关。

Geographic Variation of Sex Pheromones in Three Populations of Dendrolimus kikuchii (Lepidoptera:Lasiocampidae)

Objective] In order to unveil the mechanism of population divergences to sex pheromone responses and develop a region-specific pheromone lure for successful monitoring and control of Dendrolimus kikuchii (Lepidoptera:Lasiocampidae), we focus on the geographic variation analysis of sex pheromones of D. kikuchii according to its sex pheromone quantity, blend ratio, antennal sensitivity, and biological activity in three populations from Hunan, Jiangxi, and Yunnan Provinces. Method] Sex pheromone of D. kikuchii from different populations mentioned above was analyzed by means of Gas chromatography (GC), Electroantennographic recording (EAG), and field behavioral assays. Result] GC analyses of virgin female glands demonstrated that three pheromone components, (5Z,7E)-5,7-dodecadien-1-yl acetate (Z5,E7-12:OAc), (5Z,7E)-5,7-dodecadien-1-ol (Z5,E7-12:OH) and (5Z)-5-dodecenyl acetate (Z5-12:OAc) were present in all three populations. On average, all populations had a similar and abundant quantity of Z5,E7-12:OAc as well as trace amounts of Z5,E7-12:OH and Z5-12:OAc, the blend ratio and quantities of the three components in three populations existed relatively small differences with a blend ratio of 100:10:25 or 100:10:10 showing better EAG and behavioral activities than that of the other blend ratios. Conclusion] These results suggested that different geographic populations of D.kikuchii have developed some minor genetic variation but did not form completely independent groups. The subtle changes in sex pheromone quantities and blend ratios in different populations might be related to geographic distribution range, hosts, and man-made pesticide control interference.