| 利用AFLP和SSR标记构建美洲黑杨×青杨遗传图谱 |
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| 引用本文： | 黄秦军,苏晓华,张香华.利用AFLP和SSR标记构建美洲黑杨×青杨遗传图谱[J].林业科学研究,2004,17(3):291-299. |
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| 作者姓名： | 黄秦军 苏晓华 张香华 |
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| 作者单位： | 中国林业科学研究院林业研究所,北京,100091 |
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| 基金项目： | 国家"973"项目(G199901600004)，"863"课题(2002AA241071)资助 |
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| 摘 要： | 以美洲黑杨×青杨F2代为作图群体,利用AFLP和SSR标记技术构建分子连锁图谱。连锁图共有19个较大的连锁群,16个二联体(Doublets),7个三联体(Triplets)和5个较小的连锁群。19个较大的连锁群上包括356个AFLP标记和12个SSR标记,总图距为3382 4cM,标记间的平均间距为9 69cM。估计基因组长度为2607～3319cM,平均为3042cM,同时采用Lander和Bishop的方法计算期望基因组覆盖度,平均值分别为96 7%和98 4%。
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| 关 键 词： | 美洲黑杨×青杨 AFLP SSR 遗传图谱 |
| 文章编号： | 1001-1498(2004)03-0291-09 |
| 收稿时间： | 2004/1/12 0:00:00 |
| 修稿时间： | 2004年1月12日 |
Genetic Linkage Mapping in F2 Population of Populus deltoides×P.cathayana by Using AFLP and SSR Marker |
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| HUANG Qin-jun,SU Xiao-hua and ZHANG Xiang-hua.Genetic Linkage Mapping in F2 Population of Populus deltoides×P.cathayana by Using AFLP and SSR Marker[J].Forest Research,2004,17(3):291-299. |
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| Authors: | HUANG Qin-jun SU Xiao-hua and ZHANG Xiang-hua |
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| Institution: | Research Institute of Forestry, CAF, Beijing100091, China;Research Institute of Forestry, CAF, Beijing100091, China;Research Institute of Forestry, CAF, Beijing100091, China |
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| Abstract: | Based on the techniques of amplified fragment length polymorphism (AFLP) and microsatellite (SSR) marker, a hybrid molecular linkage map of Populus deltoides×P. cathayana was constructed. 19 major groups, 16 doublets, 7 triplets and 5 small groups were identified in this map.356 AFLP makers and 12 SSR markers, total 368 makers, were ordered on the 19 major groups. This map covered 3 382.4 cM of genome length, and the average distance between two markers was 9.69 cM. The estimated genome length was between 2 607 cM and 3 319 cM and was 3 042 cM in average. The expected genome coverage was calculated and it was 96.7% with the equation of Lander,and it was 98.4% with method of Bishop et al. |
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