| 褐环粘盖牛肝菌菌种分离研究 |
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| 引用本文： | 应国华,吕明亮,陈连庆.褐环粘盖牛肝菌菌种分离研究[J].林业科学研究,2004,17(1):66-71. |
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| 作者姓名： | 应国华 吕明亮 陈连庆 |
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| 作者单位： | 1. 浙江省丽水市林业科学研究所,浙江,丽水,323000
2. 中国林业科学研究院亚热带林业研究所,浙江,富阳,311400 |
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| 基金项目： | 2001年浙江省丽水市科技局"牛肝菌菌根成因及仿生栽培技术研究"(编号:2001003) |
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| 摘 要： | 以影响褐环粘盖牛肝菌菌种分离成活率、初始菌丝萌发速度、菌丝生长状况为参照标准,通过对培养基、分离材料、分离环境、培养方法4个方面10种因素研究表明,培养基以制作到分离时间10d的MMN配方为好,材料以阴、晴天采集的中、幼子实体的菌肉、菌管最好,分离环境可以野外就地、可以室内接种箱,培养方法以分离后试管斜放效果最好。
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| 关 键 词： | 褐环粘盖牛肝菌 菌种分离 培养基 分离材料 分离环境 培养方法 食用菌 |
| 文章编号： | 1001-1498(2004)01-0066-06 |
| 收稿时间： | 2003/2/17 0:00:00 |
A Preliminary Study on the Isolating of Suillus luteus |
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| YING Guo-hu,LU Ming-liang and CHEN Lian-qing.A Preliminary Study on the Isolating of Suillus luteus[J].Forest Research,2004,17(1):66-71. |
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| Authors: | YING Guo-hu LU Ming-liang and CHEN Lian-qing |
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| Institution: | The Forestry Research Institute of Lishui City, Zhejiang Province, Lishui323000, Zhejiang, China;The Forestry Research Institute of Lishui City, Zhejiang Province, Lishui323000, Zhejiang, China;Research Institute of Subtropicai Forestry, CAF, Fuyang311400, Zhejiang, China |
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| Abstract: | Taking the survival rate of the isolation of strain,the speed of germination of the first hypha and the state of hypha'growth as the standard,ten factors concerning the culture medium,the isolating material, the isolating environment and the culture method were studied.It showed that the medium should be ten days old from making to isolating,the best contents was BOH,the best materials were pileus and tubuli of the middle and young fructification that were collected in sunny or cloudy days.The isolating environment might be the open air or the transfer box in room.The best isolating method was to put the test tube slantways after isolating. |
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| Keywords: | Suillus luteus culture medium isolating material isolating environment culture method |
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