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| 竹伐桩促腐微生物的分离筛选 | |
| 引用本文： | 李超,李潞滨,杨凯,彭镇华.竹伐桩促腐微生物的分离筛选[J].林业科学研究,2008,21(2):253-257. |
| 作者姓名： | 李超 李潞滨 杨凯 彭镇华 |
| 作者单位： | 1. 河北大学生命科学学院,河北,保定,071002;中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 2. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 3. 北京农学院农业应用新技术北京市重点实验室,北京,102206 4. 河北大学生命科学学院,河北,保定,071002 |
| 基金项目： | 引进国际先进农业科技计划(948计划) |
| 摘 要： | 以不同腐朽程度的毛竹伐桩为样品,对其中的具有降解纤维素或木质素的竹腐微生物进行富集、分离、纯化.通过定性和定量筛选共得到16株具有较好纤维素降解能力或木质素降解能力的菌株,包括8株真菌,5株细菌和3株放线菌.采用固态竹屑培养基测定各菌株对毛竹纤维素和木质素的降解能力,真菌菌株F2和F10的降解效果最好,15 d对纤维素的降解率分别为23.96%和24.31%,优于参照菌株绿色木霉YJ-3的19.59%;对木质素的降解率分别为16.92%和19.15%,优于参照菌株黄孢原毛平革菌ME-446的16.53%. |
| 关 键 词： | 竹伐桩 微生物降解 分离筛选 纤维素 木质素 |
| 文章编号： | 1001-1498(2008)02-0253-05 |
| 收稿时间： | 2007/8/16 0:00:00 |
| 修稿时间： | 2007年8月16日 |
Isola ting and Screen ing ofM icroogan ism s for Decomposing Bamboo Stump |
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| LI Chao,LI Lu-bin,YANG Kai and PENG Zhen-hua.Isola ting and Screen ing ofM icroogan ism s for Decomposing Bamboo Stump[J].Forest Research,2008,21(2):253-257. | |
| Authors: | LI Chao LI Lu-bin YANG Kai and PENG Zhen-hua |
| Institution: | College of Life Sciences, Hebei University, Baoding 071002, Hebei, China; Research Institute of Forestry, CAF; Key Laboratory ofTree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, CAF; Key Laboratory ofTree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Key Laboratory ofNew Technology in Agricultural App lication of Beijing Agricultural College, Beijing 102206, China;Research Institute of Forestry, CAF; Key Laboratory ofTree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
| Abstract: | Some microorganism strainswhich decompose cellulose or lignin were isolated from rotten bamboo materialwhich were collected from Phyllostachys edulis forest. 16 strains including 8 fungi strains, 5 bacteria strains and 3actinomycetes strains were screened using qualitative and quantitative methods. Bamboo material decomposingability of those strainswere tested through solid fermentation. Results showed that strain F2 and F10 were the mostefficient decomposer. After 15 days of incubation, strain F2 could decompose 23. 96% of bamboo cellulose and16192% of bamboo lignin and strain F10 could decompose 24. 31% of bamboo cellulose and 23. 15% of bamboolignin. In test condition, the cellulose degradation rates of the two strains were both higher than that of theTrichoderm a viride YJ-3, and their lignin degradation rates were both higher than that of the Phanerochaetechrysosporium ME-446. |
| Keywords: | bamboo stump microbial degradation isolation and screening cellulose lignin |
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