胞外多糖和脂多糖在青枯菌对尾巨桉根部吸附和侵入过程中的作用研究

通过测定青枯菌的根表吸附量和根部侵入量,研究了胞外多糖(EPS)和脂多糖(LPS)在青枯菌对尾巨桉根部吸附和侵入过程中的作用.结果表明:在接种青枯菌的6 h内,EPS处理后尾巨桉根部青枯菌GN1和93B的吸附量和侵入量明显升高,而LPS处理后尾巨桉根部GN1和93B的吸附量和侵入量都明显下降;无EPS菌株的根表吸附量和根部侵入量都明显降低,无EPS及LPS菌株的根表吸附量和根部侵入量亦有所下降,但没有无EPS菌株下降明显;EPS具有促进青枯菌对尾巨桉根部吸附和侵入的作用,LPS则具有抑制作用.

Study on the Role of EPS and LPS of Ralstonia solanacearum in Eucalyptus urophylla × E. grandis Root Adsorption and Ingression

In this experiment, the role of extracellular polysaccharides ( EPS) and lipopolysaccharides (LPS) werestudied by detecting the number of Ralstonia solanacearum in Eucalyptus urophy lla ×E. grandis root adsorp tion andingression. The results showed that the number of GN1 and 93B in adsorp tion and ingression increased obviouslywhen the rootswere treated with EPS, while decreased evidently with LPS in 6 h after inoculation. The number ofbacteria washed off EPS decreased evidently in adsorp tion and ingression to the roots. In comparison, the number ofbacteria washed off both EPS and LPS had the same decreasing tendency, but not so evident as the former. Theseresults demonstrated that EPS p layed a role in enhancing the adsorp tion and ingression of R. solanacea rum to theroots of Eucalyptus urophy lla ×E. grandis, while LPS had inhibitory effect.