| 35杨微繁殖与叶片不定芽再生研究 |
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| 引用本文： | 何晓兰,王保松,韩杰峰,刘桂华,佘建明,倪万潮.35杨微繁殖与叶片不定芽再生研究[J].江苏林业科技,2009,36(4):1-4,16. |
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| 作者姓名： | 何晓兰 王保松 韩杰峰 刘桂华 佘建明 倪万潮 |
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| 作者单位： | 1. 江苏省农业科学院农业生物技术研究所,江苏南京,210014
2. 江苏省林业科学研究院,江苏南京,211153 |
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| 基金项目： | 江苏省科技成果转化资金项目"速生抗逆杨树、柳树新品种选育及产业化"，江苏省高技术研究计划(农业) "悬铃木不育种质的创建" |
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| 摘 要： | 以35杨茎段外植体为供试材料，对腋芽微繁殖、叶片不定芽再生、生根以及移植的离体培养技术体系进行了研究，结果表明：获得初始无菌苗的取材以4月取田间杨树新枝茎段外植体为最好；茎段外植体的启动培养基以附加NAA0．1mCL、0．5mg／L6-BA及GA，0．5mg／L的WPM培养基为优；腋芽微繁殖最快的培养基为WPM＋0．2mg／L6-BA＋0．1mg／LIAA＋0．1mg／LIBA；生根培养基以3／5WPM＋0．1mg／LIBA为好；叶片不定芽再生培养基以WPM＋0．2mg／L 6-BA＋0．1mg／LIAA＋0．1mg／LIBA再生频率最高，达92．86％；叶片不定芽在生根培养基上培养20d后获健壮生根苗，移栽成活率达100％。
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| 关 键 词： | 35杨 微繁殖 不定芽 再生 |
Micropropagation and adventitious shoot regeneration from in vitro cultured leaves of Populus deltoides L.'35/66' |
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| HE Xiao-lan,WANG Bao-song,HAN Jie-feng,LIU Gui-hua,SHE Jian-ming,NI Wan-chao.Micropropagation and adventitious shoot regeneration from in vitro cultured leaves of Populus deltoides L.'35/66'[J].Journal of Jiangsu Forestry Science & Technology,2009,36(4):1-4,16. |
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| Authors: | HE Xiao-lan WANG Bao-song HAN Jie-feng LIU Gui-hua SHE Jian-ming NI Wan-chao |
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| Institution: | 1.Institute of Agro-biological Technology;Jiangsu Academy of Agricultural Sciences;Nanjing 210014;China;2.Jiangsu Academy of Forestry;Nanjing 211153;China |
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| Abstract: | A micropropagation system was established in stem of Populus deltoides L.'35/66'.Tender stems of 35 poplar in field were been easy to sterilize in April.Axiliary buds from the stems were sprouted in WPM basal medium with 0.1 mg/L NAA,0.5 mg/L 6-BA and 0.5 mg/L GA3.Axillary buds were proliferated in WPM basal medium supplemented with 0.2 mg/L 6-BA,0.1 mg/L IAA and 0.1 mg/L IBA.Plantlets were rooted better in 3/5WPM basal medium with 0.1 mg/L IBA.Adventitious shoots were regenerated from leaf explants of root... |
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| Keywords: | Populus deltoides L '35/66' Micropropagation Adventitious bud Regeneration |
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